A highly selective optical sensor Eu-BINAM for assessment of high sensitivity cardiac troponin tumor marker in serum of cancer patients

A novel, easy, touchy and selective spectrofluorimetric technique has been successfully applied for sensitive determination of High Sensitivity Cardiac Troponin (TNHS I) in the serum samples of patients suffering malignant tumors through the usage of optical sensor Eu3+-BINAM complex. The technique is primarily based on quenching of the Eu3+-BINAM complex's luminescence intensity upon introducing various concentrations of High Sensitivity Cardiac Troponin (TNHS I). The synthesis and characterization of the optical sensor was performed via absorption and emission. The sensor was also adapted to offer excitation at 394 nm in acetonitrile at pH 7.5. Concentration of High Sensitivity Cardiac Troponin (TNHS I) in serum samples was found to be proportional to the luminescence intensity quenching of the Eu3+-BINAM complex, most prominently at lambda(em) = 618 nm. The limit of the dynamic range is 4.26 x 10(-4) to 2 ng/mL. The limit of detection and quantitation were calculated to be 1.35 and 4.10 ng/mL, respectively. The suggested analytical approach proved its applicability, simplicity and comparatively interference-free. The technique was effectively recruited to quantify High Sensitivity Cardiac Troponin (TNHS I) in human serum samples. The proposed technique could be further extended to evaluate some biomarkers associated with malignancy related diseases in human.

Automated summary

A novel, easy and selective spectrofluorimetric technique using the optical sensor Eu3+-BINAM complex was successfully applied for sensitive determination of High Sensitivity Cardiac Troponin (TNHS I) in serum samples of patients with malignant tumors. The technique is based on luminescence intensity quenching of the Eu3+-BINAM complex upon introduction of different concentrations of TNHS I. The synthesis and characterization of the optical sensor were performed, and the sensor was found to offer excitation at 394 nm in acetonitrile at pH 7.5. The concentration of TNHS I in serum samples was proportional to the luminescence intensity quenching of the Eu3+-BINAM complex, most notably at lambda(em) = 618 nm. The technique proved its applicability, simplicity, and low interference, making it effective for quantifying TNHS I in human serum samples.