One-Stop Plasmonic Nanocube-Excited SERS Immunoassay Platform of Multiple Cardiac Biomarkers for Rapid Screening and Progressive Tracing of Acute Myocardial Infarction

Rapid and precise acute myocardial infarction (AMI) diagnosis is essential for preventing patient death. In addition, the complementary roles of creatine kinase muscle brain (CK-MB) and cardiac troponin I (cTnI) cardiac biomarkers in the early and late stages of AMI demand their simultaneous detection, which is difficult to implement using conventional fluorescence and electrochemical technologies. Here, a nanotechnology-based one-stop immuno-surface-enhanced Raman scattering (SERS) detection platform is reported for multiple cardiac indicators for the rapid screening and progressive tracing of AMI events. Optimal SERS is achieved using optical property-based, excitation wavelength-optimized, and high-yield anisotropic plasmonic gold nanocubes. Optimal immunoassay reaction efficiencies are achieved by increasing immobilized antibodies. Multiple simultaneous detection strategies are implemented by incorporating two different Raman reports with narrow wavenumbers corresponding to two indicators and by establishing a computational SERS mapping process to accurately detect their concentrations, irrespective of multiple enzymes in the human serum. The SERS platform precisely estimated AMI onset and progressive timing in human serum and made rapid AMI identification feasible using a portable Raman spectrometer. This integrated platform is hypothesized to significantly contribute to emergency medicine and forensic science by providing timely treatment and observation. Rapid and precise diagnosis of acute myocardial infarction (AMI), which is associated with high mortality worldwide, is of great importance. A multipurpose surface-enhanced Raman scattering (SERS) immunoassay strategy based on incident light-optimized and optical resonance-stimulated plasmonic gold nanocubes is evaluated to detect human serum CK-MB and cTnI levels. This is applicable to single detection for lab-based quantitative analysis and multiple detections for fast judgment of AMI severity in emergencies.image

Automated summary

Rapid and precise diagnosis of acute myocardial infarction (AMI) is crucial for preventing patient death. A nanotechnology-based one-stop immuno-SERS detection platform is reported for the simultaneous detection of multiple cardiac indicators, allowing for rapid screening and tracing of AMI events.