4.7 Article

PATHPOD - A loop-mediated isothermal amplification (LAMP)-based point-of-care system for rapid clinical detection of SARS-CoV-2 in hospitals in Denmark

Related references

Note: Only part of the references are listed.
Article Multidisciplinary Sciences

Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application

Suying Lu et al.

Summary: Continued waves, new variants, and limited vaccine deployment underline the importance of SARS-CoV-2 tests in controlling the COVID-19 pandemic. This study optimized the RT-LAMP method for testing in low/medium resource settings and found it to be as sensitive as RT-PCR in detecting SARS-CoV-2. The study also demonstrated the effectiveness of a portable PoC device in replacing traditional thermocyclers for testing.

PLOS ONE (2022)

Article Multidisciplinary Sciences

Combining rapid antigen testing and syndromic surveillance improves community-based COVID-19 detection in a low-income country

Fergus J. Chadwick et al.

Summary: This study examines how combining rapid antigen testing and syndromic surveillance can improve COVID-19 detection and reduce false-positive and false-negative diagnoses in low-income communities in Dhaka, Bangladesh. The results show that this combination approach yields equal-to-better performance compared to using rapid antigen tests alone, with the best performance in the epidemic growth scenario.

NATURE COMMUNICATIONS (2022)

Article Nanoscience & Nanotechnology

Multiplexed reverse-transcriptase quantitative polymerase chain reaction using plasmonic nanoparticles for point-of-care COVID-19 diagnosis

Nicole R. Blumenfeld et al.

Summary: This study proposes a strategy using plasmonic polymerase chain reaction for multiplexed fluorescence detection of SARS-CoV-2 RNA in human samples, showing potential as a point-of-care approach. The use of small optical components enables rapid and accurate molecular clinical diagnostics in decentralized settings.

NATURE NANOTECHNOLOGY (2022)

Article Multidisciplinary Sciences

Protection of COVID-19 vaccination and previous infection against Omicron BA.1, BA.2 and Delta SARS-CoV-2 infections

Stijn P. Andeweg et al.

Summary: Protection against Omicron BA.1 and BA.2 infection is low, but booster vaccines temporarily increase protection. Previous infection, primary vaccination, and booster vaccination offer similar protection against Omicron BA.1 and BA.2 infection. Higher protection is observed in individuals with both vaccination and previous infection.

NATURE COMMUNICATIONS (2022)

Article Multidisciplinary Sciences

Detection of SARS-CoV-2 in exhaled breath from non-hospitalized COVID-19-infected individuals

Caecilie Leding et al.

Summary: SARS-CoV-2 can be detected in human breath using a non-invasive electrostatic air sampler, with the highest detection rate occurring closest to symptom onset.

SCIENTIFIC REPORTS (2022)

Article Engineering, Biomedical

A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma

Devora Najjar et al.

Summary: A 3D-printed lab-on-a-chip has been developed for rapid and accurate detection of SARS-CoV-2 RNA and antibodies, providing a convenient method for determining immune status in individuals.

NATURE BIOMEDICAL ENGINEERING (2022)

Article Engineering, Biomedical

Rapid detection of SARS-CoV-2 RNA in saliva via Cas13

Sita S. Chandrasekaran et al.

Summary: This article reports a COVID-19 testing method implemented in a microfluidic device, which can rapidly detect SARS-CoV-2 RNA in saliva samples using isothermal RNA amplification and Cas13-mediated cleavage of a quenched fluorophore. The method has high sensitivity and specificity, and the testing can be completed within 60 minutes.

NATURE BIOMEDICAL ENGINEERING (2022)

Article Biotechnology & Applied Microbiology

Point-of-care system for rapid real-time detection of SARS-CoV-2 virus based on commercially available Arduino platforms

Huynh Van Ngoc et al.

Summary: This study developed a fluorescence-based portable detection system for rapid and quantitative detection of the SARS-CoV-2 virus. The system, built on the Arduino platform, demonstrated real-time detection capability and showed excellent agreement with a commercial thermocycler. It has the potential to greatly enhance the practical applicability for on-site screening of the SARS-CoV-2 virus.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

Article Biochemistry & Molecular Biology

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

Parinaz Fozouni et al.

Summary: This study developed an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2, showing rapid, highly sensitive, and mobile phone-based readout capabilities.
Review Virology

Loop-mediated isothermal amplification (LAMP): An effective molecular point-of-care technique for the rapid diagnosis of coronavirus SARS-CoV-2

Melek Chaouch

Summary: The Covid-19 pandemic has caused significant loss worldwide. The LAMP test is considered an effective diagnostic tool for Covid-19, especially in endemic areas and low-income countries.

REVIEWS IN MEDICAL VIROLOGY (2021)

Article Biotechnology & Applied Microbiology

LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding

Kerstin U. Ludwig et al.

Summary: Frequent and fast testing with high sensitivity and specificity, low cost, and easy deployment make LAMP-Seq a promising tool for integrated public health surveillance programs in containing COVID-19 outbreaks.

NATURE BIOTECHNOLOGY (2021)

Editorial Material Biochemical Research Methods

Rapid diagnostics for SARS-CoV-2 virus: point-of-care testing and lessons learned during the pandemic Comment

Trieu Nguyen et al.

Summary: The commentary article discusses early, onsite, and rapid detection methods of SARS-CoV-2, such as point-of-care (POC) diagnostics.

BIOANALYSIS (2021)

Article Medicine, General & Internal

Characterizing long COVID in an international cohort: 7 months of symptoms and their impact

Hannah E. Davis et al.

Summary: Patients with Long COVID report prolonged, multisystem involvement and significant disability. By seven months, many patients have not yet recovered (mainly from systemic and neurological/cognitive symptoms), have not returned to previous levels of work, and continue to experience significant symptom burden.

ECLINICALMEDICINE (2021)

Article Chemistry, Analytical

Rapid molecular diagnostics of COVID-19 by RT-LAMP in a centrifugal polystyrene-toner based microdevice with end-point visual detection

Kezia Gomes de Oliveira et al.

Summary: Infection by the new coronavirus is a serious global public health issue, with mass testing being a key strategy. Loop-mediated isothermal amplification (LAMP) simplifies infectious disease diagnostics, with easy visual result detection, but caution is required to avoid contamination and false positives. LAMP on microfluidic platforms minimizes false positives and offers potential for point-of-care applications.

ANALYST (2021)

Article Medical Laboratory Technology

Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia

Daniel K. W. Chu et al.

CLINICAL CHEMISTRY (2020)

Article Medicine, General & Internal

First Case of 2019 Novel Coronavirus in the United States

Michelle L. Holshue et al.

NEW ENGLAND JOURNAL OF MEDICINE (2020)

Article Biotechnology & Applied Microbiology

CRISPR-Cas12-based detection of SARS-CoV-2

James P. Broughton et al.

NATURE BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2

Wei E. Huang et al.

MICROBIAL BIOTECHNOLOGY (2020)

Article Infectious Diseases

Serology testing in the COVID-19 pandemic response

Rosanna W. Peeling et al.

LANCET INFECTIOUS DISEASES (2020)

Review Chemistry, Analytical

Point-of-care devices for pathogen detections: The three most important factors to realise towards commercialization

Trieu Nguyen et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2020)

Article Multidisciplinary Sciences

SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification

Brian A. Rabe et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Multidisciplinary Sciences

Rapid isothermal amplification and portable detection system for SARS-CoV-2

Anurup Ganguli et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Review Medicine, Research & Experimental

MicroRNA amplification and detection technologies: opportunities and challenges for point of care diagnostics

Vivek Priy Dave et al.

LABORATORY INVESTIGATION (2019)

Article Chemistry, Analytical

From Lab on a Chip to Point of Care Devices: The Role of Open Source Microcontrollers

Trieu Nguyen et al.

MICROMACHINES (2018)

Review Health Care Sciences & Services

Infectious Disease Management through Point-of-Care Personalized Medicine Molecular Diagnostic Technologies

Luc Bissonnette et al.

JOURNAL OF PERSONALIZED MEDICINE (2012)

Article Biochemistry & Molecular Biology

Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation

Y Mori et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2001)

Article Biochemistry & Molecular Biology

Loop-mediated isothermal amplification of DNA

Tsugunori Notomi et al.

NUCLEIC ACIDS RESEARCH (2000)