Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 392, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2023.134081
Keywords
Non-enzymatic probe; Myocardial infarction; Cardiac biomarker; Glycosaminoglycan; Heparin; Biosensor; Cardiovascular disease
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In this study, positively charged green-emitting gold nanoclusters were synthesized and conjugated with heparin to serve as a fluorescence probe for the non-enzymatic detection of cardiac troponin I.
Despite the very high specificity and simplicity, antibody-based assays require high costs and top-end preservation facilities to develop an efficient biosensor. We constructed An antibody-free fluorescence probe for cardiac troponin -I, the 'gold standard' marker for diagnosing myocardial infarction. In the present work, positively charged green-emitting gold nanoclusters were synthesized through a one-pot microwave-mediated method, and the fluorescence response with heparin was studied. These heparin-conjugated gold nanoclusters were further utilized as a fluorescence probe for the non-enzymatic detection of cardiac troponin I. The linear detection of troponin with a low limit of detection of 0.1631 ng/mL was achieved with the probe. The challenging interfering perturbation of the co-existing biomolecules was evaluated, among which only glutathione showed an insignificant turn-on response within the biological limit.
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