4.7 Article

A Mitochondrial-targeted curcumin-based fluorescent probe for real-time tracking endogenous hydrogen peroxide in living cells

Journal

SENSORS AND ACTUATORS B-CHEMICAL
Volume 391, Issue -, Pages -

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2023.134039

Keywords

Fluorescent probe; Dual -channel; Near -infrared; Hydrogen peroxide; Mitochondrialtargeting

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In this study, a novel fluorescent probe DFB-1 was synthesized which showed dual-channel fluorescence response, mitochondria-targeting ability, and high signal-to-background ratio. DFB-1 could track and differentiate the fluctuation of endogenous H2O2 in hepatocellular carcinoma and colorectal cancer cells. This probe provides new insights into the function of mitochondrial endogenous H2O2.
To explore high-performance fluorescent probes that endow tunability and capability for in vivo analysis is an enduring goal of visualizing and manipulating biological events. Hydrogen peroxide H2O2, as a central redox signaling molecule, is the tough hurdle to real-time in situ tracking dynamic subcellular H2O2. Herein, we present a curcuminoid difluoroboron based fluorescent probe DFB-1 which contains the triphenylphosphine (TPP) moiety as mitochondrial targeting unit and aryl boronates as the specific uncaging group. Notably, this activatable probe DFB-1 bestows several striking characteristics: (i) dual-channel fluorescence response with a large shift; (ii) active mitochondria-targeting; (iii) light-up NIR fluorescence with high signal-to-background ratio. The probe DFB-1 is successfully employed in sensing endogenous H2O2 with a limit of detection 0.025 & mu;M, and effectively differentiate H2O2 fluctuation between hepatocellular carcinoma and colorectal cancer cells. This dual-channel NIR probe DFB-1 realizes new advances in deep insight into the function of mitochondrial endogenous H2O2.

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