The restricted mass transfer inside the anode pore channel affects the electroactive biofilms formation, community composition and the power production in microbial electrochemical systems

Porous anodes improve system performance in microbial electrochemical systems by increasing the specific surface area for electroactive bacteria. In this study, multilayer anodes with different pore diameters were constructed to assess the impact of pore size and depth on anode performance. This layered structure makes detecting electroactive biofilms more accessible layer by layer, which is the first study to examine electroactive biofilms' molecular biology and electrochemical properties at different depths in pores with varied pore sizes. The millimeter-scale pores inside the bioanode have a limited effect in increasing power. The larger the pore diameter, the higher the maximum power density (Pmax) obtained. The Pmax of anodes with 4 mm pore (1.91 & PLUSMN; 0.15 W m-2) was 1.4 times higher than that of the non-perforated (1.37 & PLUSMN; 0.07 W m-2) and 0.5 mm pore anodes (1.39 & PLUSMN; 0.04 W m-2). Electricigens can colonize into pore channels for at least 10 mm with a pore diameter & GE;3 mm and current densities >0.05 A m- 2. However, in the pores channel with 0.5 mm diameter, electricigens can only colonize to a depth of 2 mm. The biofilm thickness, electricity output, metabolic activity, and biocommunity changed with pore depth and were restricted by the limited mass transfer. The Geobacter sp. was the dominant species in inter-pore biofilms, with 43.8 %-78.6 % in abundance and decreased in quantity as pore depth increased. The inter-pore biofilms on the outer layer contributed a current density of 0.17 & PLUSMN; 0.003 A m- 2, while that of the inner layer was only 0.02 & PLUSMN; 0.01 A m- 2. Further studies found that the pore edge mass transfer effect can contribute up to 75 % of the current. The mass transfer process at the pore edge region could be a multi-directional mass transfer rather than a pore channel mass transfer.

Automated summary

Porous anodes improve system performance in microbial electrochemical systems by increasing the specific surface area for electroactive bacteria. Multilayer anodes with different pore diameters were constructed to assess the impact of pore size and depth on anode performance. The millimeter-scale pores inside the bioanode have a limited effect in increasing power, while larger pore diameters result in higher maximum power density. Electricigens can colonize into pore channels for at least 10 mm with a pore diameter ≥3 mm and current densities >0.05 A m-2. However, in the pores channel with 0.5 mm diameter, electricigens can only colonize to a depth of 2 mm. The biofilm thickness, electricity output, metabolic activity, and biocommunity changed with pore depth and were restricted by the limited mass transfer.