Neonicotinoid mixture alters trophic interactions in a freshwater aquatic invertebrate community

Neonicotinoids are increasingly and widely used systemic insecticides in agriculture, residential applications, and elsewhere. These pesticides can sometimes occur in small water bodies in exceptionally high concentrations, leading to downstream non-target aquatic toxicity. Although insects appear to be the most sensitive group to neonicotinoids, other aquatic invertebrates may also be affected. Most existing studies focus on single-insecticide exposure and very little is known concerning the impact of neonicotinoid mixtures on aquatic invertebrates at the community level. To address this data gap and explore community-level effects, we performed an outdoor mesocosm experiment that tested the effect of a mixture of three common neonicotinoids (formulated imidacloprid, clothianidin and thiamethoxam) on an aquatic invertebrate community. Exposure to the neonicotinoid mixture induced a top-down cascading effect on insect predators and zooplankton, ultimately increasing phytoplankton. Our results highlight complexities of mixture toxicity occurring in the environment that may be underestimated with traditional mono-specific toxicological approaches.

Automated summary

Neonicotinoids, widely used insecticides, can reach high concentrations in small water bodies, causing non-target aquatic toxicity. This study focused on the impact of a mixture of three neonicotinoids on an aquatic invertebrate community, revealing a cascading effect on predators and zooplankton, leading to an increase in phytoplankton. The results highlight the complexity of mixture toxicity in the environment, often underestimated in traditional toxicological approaches.