Application of Matrigel in the 3-dimension culture of female germline stem cells

Female germline stem cells (FGSCs) are a group of rare undifferentiated cells found in ovarian cortex, which have the unique ability to self-renew and differentiate. Stable maintenance and proliferation of FGSCs in culture are pivotal for clinic research. However, conventional 2-D (dimension) culture systems are limited in their ability to mimic the ovarian microenvironment during in vitro studies. To establish a suitable in vitro microenvironment for FGSCs, we conducted experiments using a Matrigel-based 3-D culture system. This involves testing different dilution ratios, medium compositions, and co-culture cells to find the optimal conditions for FGSCs maintenance and proliferation. Our results demonstrated the feasibility of using Matrigel as a FGSCs 3-D culture media. Moreover, co-culturing FGSCs with some types of cells in the Matrigel-based 3-D culture system had the potential to form ovarian organoids. Meanwhile, the safety of Matrigel was confirmed in vivo through transplantation experiment, which suggests the potential for clinic research.

Automated summary

Female germline stem cells (FGSCs) are rare undifferentiated cells in the ovarian cortex that can self-renew and differentiate. We conducted experiments using a Matrigel-based 3-D culture system to establish a suitable in vitro microenvironment for FGSCs, testing different dilution ratios, medium compositions, and co-culture cells. Our results showed that Matrigel can be used as a FGSCs 3-D culture media, and co-culturing FGSCs with certain cells in Matrigel-based system had the potential to form ovarian organoids. The safety of Matrigel was confirmed in vivo through transplantation experiment, suggesting its potential for clinical research.