Validating ASHS-T1 automated entorhinal and transentorhinal cortical segmentation in Alzheimer's disease

The current study aimed to validate entorhinal and transentorhinal cortical volumes measured by the automated segmentation tool Automatic Segmentation of Hippocampal Subfields (ASHS-T1). The study sample comprised 34 healthy controls (HCs), 37 individuals with amnestic mild cognitive impairment (aMCI), and 29 individuals with Alzheimer's disease (AD) dementia from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database. Entorhinal and transentorhinal cortical volumes were assessed using ASHS-T1, manual segmentation, as well as a widely used automated segmentation tool, FreeSurfer v6.0.1. Mean differences, intraclass correlation coefficients, and Bland-Altman plots were computed. ASHS-T1 tended to underestimate entorhinal and transentorhinal cortical volumes relative to manual segmentation and FreeSurfer. There was variable consistency and low agreement between ASHS-T1 and manual segmentation volumes. There was low-to-moderate consistency and low agreement between ASHS-T1 and FreeSurfer volumes. There was a trend toward higher consistency and agreement for the entorhinal cortex in the aMCI and AD groups compared to the HC group. Despite the differences in volume measurements, ASHS-T1 was sensitive to entorhinal and transentorhinal cortical atrophy in both early and late disease stages. Based on the current study, ASHS-T1 appears to be a promising tool for automated entorhinal and transentorhinal cortical volume measurement in individuals with likely underlying AD.

Automated summary

This study aimed to validate the measurements of entorhinal and transentorhinal cortical volumes using the automated segmentation tool ASHS-T1. ASHS-T1 tended to underestimate the volumes compared to manual segmentation and FreeSurfer. There was low agreement between ASHS-T1 and manual segmentation volumes, and low-to-moderate agreement between ASHS-T1 and FreeSurfer volumes. ASHS-T1 showed higher consistency and agreement for the entorhinal cortex in aMCI and AD groups compared to the HC group. Despite the measurement differences, ASHS-T1 was sensitive to cortical atrophy in both early and late stages of the disease.