Effect of artificially designed short peptide addition strategy on glucose oxidase activity

Charged short peptides and glucose oxidase (GOx) were studied in this paper to reveal how these peptides alter the activity and structure of GOx. Results showed that the synthesized negatively charged peptides T-8(-) and positively charged peptides T-9(+) could increase and decrease enzyme activity, respectively. The T-8(-) treated GOx potential increased by 33.6%. Meanwhile, the alpha-helix content decreased by 5.5%, and the root mean square deviation (RMSD) value decreased significantly. The fluorescence and UV-visible spectra revealed varying degrees of changes in peak and peak intensity, respectively. Overall, in the absence of contact reactions between T-9(+) and T-8(-) with GOx, the CPs changed the microenvironment around GOx and the charge arrangement on its surface, causing conformational changes in GOx and altering its enzymatic activity. The effect of CPs on GOx was further investigated using the molecular docking technique. After T-8(-) treatment, the number of hydrogen bonds increased from 3 without short peptide treatment to 8

Automated summary

This study investigated the effects of charged short peptides on the activity and structure of glucose oxidase (GOx). The results showed that negatively charged peptides increased enzyme activity, while positively charged peptides decreased it. The peptides altered the microenvironment and charge arrangement of GOx, resulting in conformational changes and altered enzymatic activity.