Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 120, Issue 33, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2303567120
Keywords
nanoparticle; nanomedicine; vaccines; drug delivery; mRNA
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Lipid nanoparticles (LNPs) are an important delivery technology that has achieved significant breakthroughs in mRNA therapeutics and vaccines. Researchers have developed a microfluidic chip to address the challenges in LNP production, achieving scalable production rates while maintaining the physical properties and efficacy of LNPs. The vaccines formulated using this chip have shown promising results in preclinical studies, demonstrating the feasibility of commercial production.
Lipid nanoparticles (LNPs) are a potent delivery technology that have made it possible for the recent clinical breakthroughs in mRNA therapeutics and vaccines. A key challenge to the broader implementation of mRNA therapeutics and vaccines is the development of technology to produce precisely defined LNP formulations, with throughput that can scale from discovery to commercial manufacturing and meet the stringent manufacturing standards of the pharmaceutical industry. To address these challenges, we have developed a microfluidic chip that incorporates 1x, 10x, or 256x LNP-generating units that achieve scalable production rates of up to 17 L/h of precisely defined LNPs. Using these chips, we demonstrate that LNP physical properties and potency in vivo are unchanged as throughput is scaled. Our chips are fabricated out of silicon and glass substrates, which have excellent solvent compatibility, compatibility with pharmaceutical manufacturing, and can be fully reset and reused. SARS-CoV- 2 mRNA-LNP vaccines formulated by our chips triggered potent antibody responses in a preclinical study. These results demonstrate the feasibility of directly translating microfluidic-generated LNPs to the scale necessary for commercial production.
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