Journal
MOLECULES
Volume 21, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/molecules21091211
Keywords
quorum sensing; quorum sensing inhibition; Chromobacterium violaceum; screening; flavonoids; flavones; violacein quantification; assay optimization; anti-biofilm; microcolonies
Funding
- Academy of Finland [272266]
- Svenska Tekniska Vetenskapsakademien i Finland
- Academy of Finland (AKA) [272266, 272266] Funding Source: Academy of Finland (AKA)
Ask authors/readers for more resources
Quorum sensing (QS) is the process by which bacteria produce and detect signal molecules to coordinate their collective behavior. This intercellular communication is a relevant target for anti-biofilm therapies. Here we have optimized a screening-applicable assay to search for new quorum sensing inhibitors from natural compound libraries. In this system, QS is correlated with the production of violacein, which is directly controlled by the LuxI/LuxR system in Chromobacterium violaceum ATCC 31532. The parallel use of C. violaceum Tn5-mutant CV026, which depends on auto-inducer addition, allows simultaneous discrimination of compounds that act as quenchers of the AHL signal (quorum quenchers). The incorporation of a redox stain into the platform allowed further distinction between QS inhibitors, quorum quenchers and antibacterial compounds. A pilot screening was performed with 465 natural and synthetic flavonoids. All the most active compounds were flavones and they displayed potencies (IC50) in the range of 3.69 to 23.35 M. These leads were particularly promising as they inhibited the transition from microcolonies into mature biofilms from Escherichia coli and Pseudomonas aeruginosa strains. This approach can be very effective in identifying new antimicrobials posing lesser risks of resistance.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available