Effect of trans-cinnamaldehyde nanoemulsion wash on chicken embryo development in fertilized eggs

Cleanliness of eggs is critical in successful hatching egg operations. The objective of this study was to investigate the effect of trans-cinnamaldehyde nanoemul-sion (TCNE) wash treatments, as a sanitation strategy, on embryonic development in fertilized eggs. Trans-cinna-maldehyde is a generally recognized as safe status phyto-chemical obtained from cinnamon bark. TCNE were prepared with emulsifiers Tween 80 (Tw.80) or gum Ara-bic and lecithin (GAL) by sonication. Day-old fertilized eggs were subjected to TCNE wash treatments at 34 & DEG;C for 5 min, followed by 18 d of incubation at 37.7 & DEG;C. Washing of fertilized eggs with TCNE-Tw.80 or GAL at 0.48% con-centration did not significantly alter the egg weight at d 18 of incubation, as compared to baseline and control (P > 0.05). The egg weight loss (calculated as percentage) did not differ significantly between eggs subjected to nanoe-mulsion wash treatments and control eggs (P > 0.05). In case of embryo fertility and mortality, for baseline and con-trol, & drangbrac; 95% fertility rate was achieved, with combined early and midterm mortality at 16%. Likewise, TCNE-Tw.80 or TCNE-GAL resulted in 95% fertility (P > 0.05), with 11% and 17% combined early and midterm mortality, respectively. Furthermore, TCNE wash treatments did not differ significantly in yolk sac and embryo weight (as com-pared to control) and did not affect the length of the d 18 embryo (P > 0.05). Moreover, TCNE wash treatments did not alter tibia weight and length (P > 0.05). Results sug-gest that TCNE could potentially be used as a natural antimicrobial for fertilized egg sanitation. Further studies in industry settings are warranted.

Automated summary

The effect of TCNE wash treatments on embryonic development in fertilized eggs was investigated. Results showed that washing with TCNE-Tw.80 or TCNE-GAL did not significantly affect egg weight, fertility rate, and mortality rate. Additionally, TCNE wash treatments did not alter yolk sac and embryo weight, as well as tibia weight and length.