Detection of CD36 gene polymorphism associated with chicken carcass traits and skin yellowness

Investigations into the association between chicken traits and genetic variations provide helpful breeding information to improve production performance and economic benefits in chickens. The single nucleotide polymorphism technique is an impor-tant method in agricultural molecular breeding. In this study, we detected 11 SNPs in the CD36 gene, 2 SNPs (g.-1974 A>G, g.-1888 T>C) located in the 50 flanking regions, 8 SNPs (g.23496 G>A, g.23643 C>T, g.23931 T>C, g.23937 G>A, g.31256 C>A, g.31258 C>T, g.31335 C>T, g.31534 A>C) located in the intron region, 1 SNPs (g.23743 G>T) located in the exon region and it belongs to synonymous mutation. In SNPs g.23743 G>T, the abdominal fat weight and abdominal fat weight rate of the GG genotype were lower than that of the TT genotype. In SNPs g.23931 T>C, the full-bore weight rate and half-bore weight rate of the TT genotype were higher compared with the CC genotype. And the SNPs g.-1888 T>C, g.23496 G>A, g.23643 C>T, g.31335 C>T and g.31534 A>C were significantly associated with skin yellowness traits, the cloacal skin yellowness before slaughter of the TT genotype was higher than that of the TC and CC geno-type in SNPs g.-1888 T>C. Furthermore, 3 haplotypes of the above eleven SNPs were calculated and they cor-related with heart weight, stomach weight, wing weight, leg skin yellowness and shin skin yellowness before slaughter. Finally, the CD36 expression profile displayed the expression pattern of CD36 mRNA varia-tion in different tissues.

Automated summary

Investigations into the association between chicken traits and genetic variations provide helpful breeding information. The single nucleotide polymorphism technique is widely used in agricultural breeding.