Multiple challenges in the development of commercial crops using CRISPR/ Cas technology

The CRISPR/Cas system is a highly efficient and versatile tool for editing plant genomes, with the potential to accelerate breeding programs and improve the sustainability of food production. Nevertheless, technical limitations delay the rapid spread of the CRISPR/Cas system benefits in agriculture. The natural features of plant species, including reproductive behavior, ploidy levels, genetic diversity, and generation times, can significantly impact the introgression of edited traits into elite germplasms. The production and selection of edited events require the same level of effort as those of their transgenic equivalents. Additionally, edited alleles tend to be recessive or not fully dominant, which differs from dominant transgenic events. To accelerate the introgression of edited events into conventional and transgenic varieties, we suggest utilizing edits on single-copy genes that induce dominant mutations. In the absence of new, simple traits that provide exceptional economic benefits for large companies, like herbicide tolerance in transgenic crops, we propose the emergence of particular public grants for edited variety productions, especially when the introgression shows a high level of technical feasibility. In the context of climate change, these public actions must be taken quickly to alleviate significant reductions in crop production.

Automated summary

The CRISPR/Cas system is an efficient tool for editing plant genomes, but technical limitations and the unique characteristics of plants impact the spread of its benefits in agriculture. Effort and selection for edited traits are comparable to transgenic equivalents, and dominant mutations on single-copy genes may accelerate their integration into varieties. Public grants for edited variety productions can be considered to boost feasibility and address challenges in crop production due to climate change.