Pantoea conspicua promoted sunflower growth and engulfed rhizospheric arsenate by secreting exopolysaccharide

A rhizobacterium, Pantoea conspicua, was examined against sunflower seedlings' growth under arsenate stress. Sunflower upon exposure to arsenate resulted in compromised growth that might be due to the accumulation of higher concentrations of arsenate and reactive oxygen species (ROS) in seedlings' tissues. The deposited arsenate led to oxidative damage and electrolyte leakage, making the sunflower seedlings vulnerable to compromise its growth and development. However, inoculation of sunflower seedlings with P. conspicua alleviated arsenate stress in host by initiating a multilayered defence mechanism. In fact, P. conspicua filtered out 75.1% of the arsenate from growth medium that were available to the plant roots in the absence of the said strain. To accomplish such activity, P. conspicua secreted exopolysaccharides as well as altered lignification in host roots. The arsenate (24.9%) that made its way to plant tissues was countered by helping the host seedlings to produce higher levels of indole acetic acid, non enzymatic antioxidants (phenolics and flavonoids) and antioxidant en-zymes (catalase, ascorbte peroxidase, peroxidase, superoxide dismutase). As a result, ROS accumulation and electrolyte leakage were brought back to normal levels as observed in control seedlings. Hence, the rhizo-bacterium associated host seedlings achieved higher net assimilation (127.7%) and relative growth rate (113.5%) under 100 ppm of arsenate stress. The work concluded that P. conspicua alleviated arsenate stress in the host plants by imposing physical barrier as well as improving host seedlings' physiology and biochemistry.

Automated summary

The rhizobacterium Pantoea conspicua was found to promote the growth of sunflower seedlings under arsenate stress. Arsenate exposure compromised sunflower growth due to the accumulation of higher concentrations of arsenate and reactive oxygen species (ROS) in the seedlings' tissues. However, inoculation of the seedlings with P. conspicua alleviated arsenate stress by implementing a multilayered defence mechanism. P. conspicua removed 75.1% of the arsenate from the growth medium and helped the seedlings produce higher levels of antioxidants, resulting in improved growth and reduced oxidative damage.