Coordinated gene upregulation in maize through CRISPR/Cas-mediated enhancer insertion

Article Biochemistry & Molecular Biology

BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize

Christian Damian Lorenzo et al.

Summary: Ensuring food security while adapting to climate change is the main challenge for agriculture in the 21st century. Recent advances in CRISPR/Cas9 gene engineering have provided a way to accelerate plant breeding to meet the increasing demand. The gene discovery pipeline BREEDIT combines multiplex genome editing with crossing schemes to improve complex traits such as yield and drought tolerance.

PLANT CELL (2023)

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Article Agronomy

CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products

Michael L. Nuccio et al.

Summary: CRISPR-Cas technologies have revolutionized maize genetics research since 2012, with targeted mutagenesis being a key application for confirming candidate genes. Ongoing developments aim to overcome challenges in target gene identification, guide RNA development, vector design, reagent delivery, and plant characterization, leading to the creation of novel breeding tools and demonstration of how CRISPR-Cas technologies can enhance maize germplasm.

MOLECULAR BREEDING (2021)

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Article Plant Sciences

Improving Nitrogen Use Efficiency Through Overexpression of Alanine Aminotransferase in Rice, Wheat, and Barley

Jingwen Tiong et al.

Summary: Nitrogen is essential for plants, but crop plants are inefficient in using applied nitrogen, leading to overuse of nitrogen fertilizers. Transgenic rice over-expressing HvAlaAT showed increased above-ground biomass with little change in nitrogen uptake rates. The study revealed that higher metabolic turnover, upregulation of glycolysis and TCA cycle genes, and involvement of phytohormonal responses may contribute to the nitrogen-use efficient phenotype. Additionally, the transgenic crop plants increased seed production under controlled environmental conditions, indicating the potential of HvAlaAT in improving nitrogen utilization efficiency.

FRONTIERS IN PLANT SCIENCE (2021)

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Article Biotechnology & Applied Microbiology

Targeted, efficient sequence insertion and replacement in rice

Yuming Lu et al.

NATURE BIOTECHNOLOGY (2020)

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Article Biochemistry & Molecular Biology

Genome-Scale Sequence Disruption Following Biolistic Transformation in Rice and Maize

Jianing Liu et al.

PLANT CELL (2019)

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Article Biotechnology & Applied Microbiology

Transgenic expression of plastidic glutamine synthetase increases nitrogen uptake and yield in wheat

Mengyun Hu et al.

PLANT BIOTECHNOLOGY JOURNAL (2018)

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Coordinated gene upregulation in maize through CRISPR/Cas-mediated enhancer insertion

Related references

BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize

CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products

Improving Nitrogen Use Efficiency Through Overexpression of Alanine Aminotransferase in Rice, Wheat, and Barley

Targeted, efficient sequence insertion and replacement in rice

Genome-Scale Sequence Disruption Following Biolistic Transformation in Rice and Maize

Transgenic expression of plastidic glutamine synthetase increases nitrogen uptake and yield in wheat

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