Journal
PHYTOCHEMISTRY
Volume 214, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2023.113795
Keywords
Psammosilene tunicoides; Caryophyllaceae; Triterpenoid saponins; Aglycone; Analgesic effect
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Five undescribed oleanane-type triterpenoid saponins, psammosaponins A-E, were isolated from the roots of Psammosilene tunicoides, along with nine known compounds. Three aglycones were also isolated from the acid-hydrolyzed extract of P. tunicoides. The structures of all compounds were determined through extensive analysis and various experiments. These compounds were evaluated for their antinociceptive activity and showed significant inhibitory effects on mouse writhing response.
Herein, five undescribed oleanane-type triterpenoid saponins, namely, psammosaponins A-E, along with nine known compounds, were isolated from the roots of Psammosilene tunicoides. Moreover, part of the ethanolic extract of P. tunicoides was acid-hydrolyzed and three aglycones were isolated from the resulting hydrolysate. The structures of all compounds were established through extensive analysis involving 1D and 2D NMR experiments, HRESIMS measurements, chemical derivatization, and comparison of spectroscopic data with the values reported in the literature. In all, 10 of the isolated saponins and the three aglycones were evaluated in the acetic acidinduced writhing model for their antinociceptive activity. At a dose of 40 mg/kg, these compounds exhibited significant inhibitory effects on the mouse writhing response, with inhibitions ranging from 31.9% to 79.3%. In addition, the structure-activity relationships of the isolates were discussed. Among the isolates, quillaic acid 3-Oglucuronide and 16 & alpha;-hydroxygypsogenic acid showed better antinociceptive activity with inhibitions of 79.3% and 73.7%, respectively. Both isolates also exhibited antinociceptive activities in hot plate and formalin tests on mice. Their antinociceptive mechanism was explored in lipopolysaccharide-stimulated RAW 264.7 cells. These isolates could significantly inhibit the production of nitric oxide and interleukin-6 and downregulate the expression levels of inducible NO synthase, COX-1, and COX-2.
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