Journal
MOLECULES
Volume 21, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/molecules21070919
Keywords
biocatalysis; industrial biotechnology; silk; biosensors; de novo engineering
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This commentary outlines a protein engineering approach as an alternative to immobilisation developed in our laboratory. We use a recombinant silk protein into which metal active sites can be incorporated to produce solid-state metalloprotein materials. The silk protein directly coordinates to the metal centres providing control over their reactivity akin to that seen in naturally occurring metalloproteins. These solid-state materials are remarkably stable at a range of temperatures and different solvent conditions. I discuss the genesis of this approach and highlight areas where such solid-state materials could find application.
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