Journal
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
Volume -, Issue -, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00424-023-02865-1
Keywords
Imaging; Calcium; Sodium; Neuron; Ion channels; Axon; Dendrite; Spine
Categories
Ask authors/readers for more resources
This review examines the importance of using low-affinity fluorescent indicators to measure the dynamics of Ca2+ or Na+ concentration. It highlights the ability to obtain spatial and temporal information with little perturbation and its potential applications in neurophysiological studies.
Low-affinity fluorescent indicators for Ca2+ or Na+ allow measuring the dynamics of intracellular concentration of these ions with little perturbation from physiological conditions because they are weak buffers. When using synthetic indicators, which are small molecules with fast kinetics, it is also possible to extract spatial and temporal information on the sources of ion transients, their localization, and their disposition. This review examines these important aspects from the biophysical point of view, and how they have been recently exploited in neurophysiological studies. We first analyze the environment where Ca2+ and Na+ indicators are inserted, highlighting the interpretation of the two different signals. Then, we address the information that can be obtained by analyzing the rising phase and the falling phase of the Ca2+ and Na+ transients evoked by different stimuli, focusing on the kinetics of ionic currents and on the spatial interpretation of these measurements, especially on events in axons and dendritic spines. Finally, we suggest how Ca2+ or Na+ imaging using low-affinity synthetic fluorescent indicators can be exploited in future fundamental or applied research.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available