Journal
PEPTIDES
Volume 166, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2023.171037
Keywords
Prolactin; Neuroprotection; Excitotoxicity; PI3K; AKT; Hippocampal neurons
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The aim of this study was to evaluate the signaling pathways involved in prolactin (PRL) neuroprotection against excitotoxicity. The results showed that PRL activated the PI3K/AKT signaling pathway, promoting neuronal survival and activating GSK3 β/NF-κB, resulting in upregulation of Bcl-2 and Nrf2 gene expression. These findings support the potential use of PRL as a neuroprotective agent in various neurological and neurodegenerative diseases.
Prolactin (PRL) is a polypeptide hormone that has been reported to play a significant role in neuroprotection against neuronal excitotoxicity produced by glutamate (Glu) or kainic acid (KA) in both, in vitro and in vivo models. However, the molecular mechanisms involved in PRL's neuroprotective effects in the hippocampus have not been completely elucidated. The aim of the present study was to assess the signaling pathways involved in PRL neuroprotection against excitotoxicity. Primary rat hippocampal neuronal cell cultures were used to assess PRL-induced signaling pathway activation. The effects of PRL on neuronal viability, as well as its effects on activation of key regulatory pathways, phosphoinositide 3-kinases/Protein Kinase B (PI3K/AKT) and glycogen synthase kinase 3 & beta; / nuclear factor kappa B (GSK3 & beta;/NF-& kappa;B), were evaluated under conditions of Glutamateinduced excitotoxicity. Additionally, the effect on downstream regulated genes such as Bcl-2 and Nrf2, was assessed. Here, we show that the PI3K/AKT signaling pathway is activated by PRL treatment during excitotoxicity, promoting neuronal survival through upregulation of active AKT and GSK3 & beta;/NF-& kappa;B, resulting in induction of Bcl-2 and Nrf2 gene expression. Inhibition of the PI3K/AKT signaling pathway abrogated the protective effect of PRL against Glu-induced neuronal death. Overall, results indicate that the neuroprotective actions of PRL are mediated in part, by the activation of the AKT pathway and survival genes. Our data support the idea that PRL could be useful as a potential neuroprotective agent in different neurological and neurodegenerative diseases.
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