Journal
PANCREATOLOGY
Volume 23, Issue 6, Pages 663-673Publisher
ELSEVIER
DOI: 10.1016/j.pan.2023.07.008
Keywords
Pancreatic ductal adenocarcinoma; PDAC; KPC mouse model; Microbiome; Oxford nanopore technologies
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The study found similarities in the microbial composition of tumor microbiomes between pancreatic ductal adenocarcinoma (PDAC) mice and human PDAC patients. KPC tumors harbored significantly more bacterial components compared to healthy pancreas tissue.
Background: Emerging evidence has recently revealed a prominent role of the microbiome in pancreatic ductal adenocarcinoma (PDAC). However, while most observations were made in patients, mouse models still require a precise characterization of their disease-related microbiome to employ them for mechanistic and interventional preclinical studies. Methods: To investigate the fecal and tumoral microbiome of LSL-Kras(G12D/+);LSL-Trp53(R172H/+);Pdx-1-Cre (KPC) and control (CTRL) mice, Oxford Nanopore sequencing was applied. Feces were collected from 10 KPC mice and 10 CTRLs at 3 timepoints (6 weeks, 12 weeks, and when tumor-bearing (KPC) or 6 months (CTRL), respectively). Metagenomic sequencing was performed on feces DNA. KPC tumor and healthy pancreas DNA samples were subjected to 16S rRNA gene sequencing. Bacterial marker components were detected in KPC tumor tissue over time by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC).Results: Murine fecal samples showed a significantly different microbiome compared to age-matched healthy CTRLs regarding beta diversity (p = 0.001, R2 = 0.2-0.25 for Bray-Curtis). Adjusted human PDAC classifiers predicted disease status from feces of KPC mice achieving area under the receiver operating characteristic (AUROC) values of 80%. Furthermore, KPC tumors harbored significantly more bacterial components than healthy pancreas. Also the microbial composition differs significantly between KPC tumors and healthy pancreas tissue (p = 0.042 for Bray-Curtis). Microbiota found highly abundant in human PDAC samples were considerably more abundant in KPC tumors as compared to healthy pancreas samples (p-value <0.001).Conclusion: KPC fecal samples show similarities with the microbial composition of stool samples from human PDAC patients.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of IAP and EPC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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