Journal
MOLECULAR REPRODUCTION AND DEVELOPMENT
Volume 83, Issue 6, Pages 488-496Publisher
WILEY
DOI: 10.1002/mrd.22644
Keywords
-
Funding
- Japan Science and Technology Agency (JST)
- Ministry of Education, Culture, Sport, Science
- Leading Advanced Projects for medical innovation (LEAP)
- AMED
Ask authors/readers for more resources
Round spermatid injection (ROSI) into unfertilized oocytes enables a male with a severe spermatogenesis disorder to have children. One limitation of the application of this technique in the clinic is the identification and isolation of round spermatids from testis tissue. Here we developed an efficient and simple method to isolate rodent haploid round spermatids using flow cytometric cell sorting, based on DNA content (stained with Hoechst 33342 or Dye Cycle Violet) or by cell diameter and granularity (forward and side scatter). ROSI was performed with round spermatids selected by flow cytometry, and we obtained healthy offspring from unstained cells. This non- invasive method could therefore be an effective option for breeding domestic animals and human male infertility treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available