4.8 Article

Click display: a rapid and efficient in vitro protein display method for directed evolution

Related references

Note: Only part of the references are listed.
Article Biotechnology & Applied Microbiology

A Multi-Specific DARPin Potently Neutralizes Shiga Toxin 2 via Simultaneous Modulation of Both Toxin Subunits

Yu Zeng et al.

Summary: Shiga toxin-producing E. coli (STEC) is a common cause of bloody diarrhea and can lead to a severe complication called hemolytic uremic syndrome (HUS). Currently, there is no effective treatment for HUS. This study reports the engineering of a new type of protein called designed ankyrin repeat proteins (DARPin), which can effectively neutralize the major subtype of Shiga toxin associated with HUS. The study also found that DARPin can protect mice from toxin damage by interfering with the toxin's activity and binding ability.

BIOENGINEERING-BASEL (2022)

Article Biotechnology & Applied Microbiology

PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high-throughput sequencing

Sabrina Galinanes Reyes et al.

Summary: The refined PURE-based display method was developed for the preparation of mRNA and cDNA-peptide conjugates, showing efficient enrichment of FLAG epitope motifs, especially in mRNA display. The study demonstrated that gel purification steps improve conjugate formation efficiency and enhance the enrichment rate of specific epitope motifs. Overall, the research suggests the potential of the PURE system for exploring diverse polypeptide sequences and functional space in future studies.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

Article Multidisciplinary Sciences

Highly accurate protein structure prediction with AlphaFold

John Jumper et al.

Summary: Proteins are essential for life, and accurate prediction of their structures is a crucial research problem. Current experimental methods are time-consuming, highlighting the need for accurate computational approaches to address the gap in structural coverage. Despite recent progress, existing methods fall short of atomic accuracy in protein structure prediction.

NATURE (2021)

Article Multidisciplinary Sciences

Highly accurate protein structure prediction for the human proteome

Kathryn Tunyasuvunakool et al.

Summary: Using the AlphaFold method, the structural coverage of the proteome has been significantly expanded, covering 98.5% of human proteins with 58% of residues having confident predictions and 36% having very high confidence. Introducing new metrics to interpret the dataset and identify disordered regions, this study aims to provide high-quality predictions for generating biological hypotheses.

NATURE (2021)

Article Chemistry, Multidisciplinary

cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins

Taishi Kondo et al.

Summary: Researchers developed a cDNA TRAP display for rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker, a monobody was covalently attached to the cDNA, and a proof-of-concept was demonstrated with the selection of an anti-EGFR1 monobody in a solution containing ribonuclease.

CHEMICAL COMMUNICATIONS (2021)

Article Biochemical Research Methods

In vitro selection of anti-gliadin single-domain antibodies from a naive library for cDNA-display mediated immuno-PCR

Chathuni Jayathilake et al.

ANALYTICAL BIOCHEMISTRY (2020)

Review Biochemical Research Methods

In Vitro Selection of Peptides and Proteins-Advantages of mRNA Display

Matilda S. Newton et al.

ACS SYNTHETIC BIOLOGY (2020)

Article Multidisciplinary Sciences

Antibody-like proteins that capture and neutralize SARS-CoV-2

T. Kondo et al.

SCIENCE ADVANCES (2020)

Review Chemistry, Multidisciplinary

Biomedical applications of copper-free click chemistry: in vitro, in vivo, and ex vivo

Eunha Kim et al.

CHEMICAL SCIENCE (2019)

Article Multidisciplinary Sciences

SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation

Wei Shen et al.

PLOS ONE (2016)

Article Biotechnology & Applied Microbiology

A versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display

Yuki Mochizuki et al.

JOURNAL OF BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

Quick, Selective and Reversible Photocrosslinking Reaction between 5-Methylcytosine and 3-Cyanovinylcarbazole in DNA Double Strand

Kenzo Fujimo et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2013)

Article Chemistry, Multidisciplinary

TRAP Display: A High-Speed Selection Method for the Generation of Functional Polypeptides

Takahiro Ishizawa et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Chemistry, Applied

Streamlined Protocol for mRNA Display

Pamela A. Barendt et al.

ACS COMBINATORIAL SCIENCE (2013)

Article Biochemical Research Methods

FLASH: fast length adjustment of short reads to improve genome assemblies

Tanja Magoc et al.

BIOINFORMATICS (2011)

Article Biochemical Research Methods

mRNA display for the selection and evolution of enzymes from in vitro-translated protein libraries

Burckhard Seelig

NATURE PROTOCOLS (2011)

Article Biochemical Research Methods

Ribosome display:: selecting and evolving proteins in vitro that specifically bind to a target

Christian Zahnd et al.

NATURE METHODS (2007)

Review Biochemical Research Methods

In-vitro protein evolution by ribosome display and mRNA display

D Lipovsek et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2004)

Article Biotechnology & Applied Microbiology

Cell-free translation reconstituted with purified components

Y Shimizu et al.

NATURE BIOTECHNOLOGY (2001)