Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 60, Issue 7, Pages 1615-1624Publisher
WILEY
DOI: 10.1002/mnfr.201500950
Keywords
Gene expression; Maternal nutrition; Nutrigenomics; Pregnancy; Pyridoxine
Categories
Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/20441-9, 2014/01288-0]
- Conselho Nacional para o Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/20441-9] Funding Source: FAPESP
Ask authors/readers for more resources
ScopeVitamin B-6 plays crucial roles on brain development and its maternal deficiency impacts the gamma-aminobutyric acid (GABA)ergic, serotonergic, glutamatergic, and dopaminergic systems in offspring. However, the molecular mechanisms underlying these neurological changes are not well understood. Thus, we aimed at evaluating which components of those neurotransmitter metabolism and signaling pathways can be modulated by maternal vitamin B-6-deficient or B-6-supplementated diets in the hippocampus of rat dams and their offspring. Methods and resultsFemale Wistar rats were fed three different diets: control (6 mg vitamin B-6/kg), supplemented (30 mg vitamin B-6/kg) or deficient diet (0 mg vitamin B-6/kg), from 4 weeks before pregnancy through lactation. Newborn pups (10 days old) from rat dams fed vitamin B-6-deficient diet presented hyperhomocysteinemia and had a significant increase in mRNA levels of glutamate decarboxylase 1 (Gad1), fibroblast growth factor 2 (Fgf2), and glutamate-ammonia ligase (Glul), while glutaminase (Gls) and tryptophan hydroxylase 1 (Tph1) mRNAs were downregulated. Vitamin B-6 supplementation or deficiency did not change hippocampal global DNA methylation. ConclusionA maternal vitamin B-6-deficient diet affects the expression of genes related to GABA, glutamate, and serotonin metabolisms in offspring by regulating Gad1, Glul, Gls, and Tph1 mRNA expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available