Journal
NANOMEDICINE
Volume 18, Issue 13, Pages 945-960Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2023-0076
Keywords
cervical cancer; gene therapy; gold nanoparticles; immunotherapy; IL-12; nanomedicine
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The aim of this study is to synthesize curcumin-reduced gold nanoparticles (AuNPs) for efficient delivery and expression of the IL-12 gene in cervical cancer (HeLa) cells in vitro. Stable, spherical AuNPs effectively compacted and protected the IL-12 DNA and tolerated well in vitro. Real-time quantitative PCR and ELISA confirmed the successful delivery and expression of the IL-12 gene in HeLa cells. The favorable attributes of this AuNP-delivery system and the significant IL-12 expression obtained suggest potential for cytokine-based therapy or immunotherapy in cervical cancer.
Aim: To synthesize curcumin-reduced gold nanoparticles (AuNPs) for the efficient delivery to and expression of the IL-12 gene in cervical cancer (HeLa) cells in vitro. Methods: Curcumin-reduced AuNPs were synthesized, stabilized with poly-L-lysine and PEG, conjugated to IL-12 DNA and physicochemically characterized. Cytotoxicity and IL-12 expression were accessed in vitro. Results & discussion: Stable, spherical AuNPs effectively compacted and protected the IL-12 DNA and tolerated well in vitro. Real-time quantitative PCR and ELISA confirmed the successful delivery and expression of the IL-12 gene in HeLa cells. Conclusion: The favorable attributes of this AuNP-delivery system and the significant IL-12 expression obtained augur well for cytokine-based therapy or immunotherapy in cervical cancer.
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