Journal
MOLECULAR MICROBIOLOGY
Volume 103, Issue 1, Pages 55-66Publisher
WILEY
DOI: 10.1111/mmi.13541
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Funding
- Bundesministerium fur Bildung und Forschung (BMBF) within the BioProFi program 'ESE-BIOGAS' [03SF0424A]
- program 'BioInterfaces in Technology and Medicine BIF-TM' of the Helmholtz Association
- Fundacao para a Ciencia e Tecnologia (FCT) - FEDER funds [LISBOA-01-0145-FEDER-007660, UID/CBQ/04612/2013]
- FCT [PTDC/BIA-MIC/1399/2014]
- European Union [635536]
- Fundação para a Ciência e a Tecnologia [UID/CBQ/04612/2013] Funding Source: FCT
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Propionate is an abundant carboxylic acid in nature. Microorganisms metabolize propionate aerobically via the 2-methylcitrate pathway. This pathway depends on a series of three reactions in the citric acid cycle that leads to the conversion of succinate to oxaloacetate. Interestingly, the c-proteobacterium Escherichia coli can use propionate as a carbon and electron source under oxic but not under anoxic conditions. RT-PCR and transcriptomic analysis revealed a posttranscriptional regulation of the prpBCDE-gene cluster encoding the necessary enzymes for propionate metabolism. The polycistronic mRNA seems to be hydrolyzed in the 30-50 direction under anoxic conditions. This regulatory strategy is highly constructive because the last gene of the operon encodes the first enzyme of the propionate metabolism. Further analysis revealed that RNase R is involved in the hydrolysis of the prp transcripts. Consequently, an rnr-deletion strain could metabolize propionate under anoxic conditions. To the best of our knowledge, this is the first study describing the influence of RNase R on the anaerobic metabolism of E. coli.
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