Journal
MOLECULAR MICROBIOLOGY
Volume 99, Issue 6, Pages 1134-1148Publisher
WILEY
DOI: 10.1111/mmi.13291
Keywords
-
Categories
Funding
- FP7 EC Kaladrug-R project [222895]
- Wellcome Trust
- Wellcome Trust Sanger Institute [098051]
- BBSRC Research Experience Placement [BB/J014540/1]
- Wellcome Trust [101239/Z/13/Z]
- National Science Foundation [NSF PHY11-25915]
- Belgian Science Policy Office (TRIT) [P7/41]
- Royal Society
- Wellcome Trust [101239/Z/13/Z] Funding Source: Wellcome Trust
Ask authors/readers for more resources
In this study, we followed the genomic, lipidomic and metabolomic changes associated with the selection of miltefosine (MIL) resistance in two clinically derived Leishmania donovani strains with different inherent resistance to antimonial drugs (antimony sensitive strain Sb-S; and antimony resistant Sb-R). MIL-R was easily induced in both strains using the promastigote-stage, but a significant increase in MIL-R in the intracellular amastigote compared to the corresponding wild-type did not occur until promastigotes had adapted to 12.2 M MIL. A variety of common and strain-specific genetic changes were discovered in MIL-adapted parasites, including deletions at the LdMT transporter gene, single-base mutations and changes in somy. The most obvious lipid changes in MIL-R promastigotes occurred to phosphatidylcholines and lysophosphatidylcholines and results indicate that the Kennedy pathway is involved in MIL resistance. The inherent Sb resistance of the parasite had an impact on the changes that occurred in MIL-R parasites, with more genetic changes occurring in Sb-R compared with Sb-S parasites. Initial interpretation of the changes identified in this study does not support synergies with Sb-R in the mechanisms of MIL resistance, though this requires an enhanced understanding of the parasite's biochemical pathways and how they are genetically regulated to be verified fully.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available