Annona cherimola Seed Extracts Trigger an Early Apoptosis Response and Selective Anticlonogenic Activity against the Human Gastric Carcinoma Cell Line SNU-1

The aim of this study was to evaluate, for the first time, the antiproliferative, apoptotic and diminishing effects of the anchored growth-independent capacity of an ethanol macerate extract from the Annona cherimola seed (EMCHS) in the human gastric cancer cell line SNU-1. The cells treated with EMCHS (20 mu g/mL) significantly reduced the capacity to form clones of the tumor cell. Moreover, 50 mu g/mL of EMCHS extract induced apoptosis, as was shown by the Annexin-V assay. UHPLC-MS/MS analysis detected two acetogenins (Annonacinone and Annonacin) in the EMCHS, which could be largely responsible for its selective antiproliferative effect. The identification of fatty acids by GC-FID showed the presence of eight fatty acids, among which was, oleic acid, which has recognized activity as an adjuvant in antitumor treatments. Taken together, our results indicate that the EMCHS seems promising for use as a natural therapy against gastric cancer disease.

Automated summary

This study evaluated the antiproliferative and apoptotic effects of an ethanol macerate extract from Annona cherimola seed (EMCHS) in human gastric cancer cells. EMCHS reduced the tumor cell cloning capacity and induced apoptosis. UHPLC-MS/MS analysis identified two acetogenins as potential key components responsible for the selective antiproliferative effect of EMCHS. In addition, GC-FID analysis detected the presence of fatty acids, including oleic acid, which has known antitumor activity. Thus, EMCHS shows potential as a natural therapy for gastric cancer.