Journal
MOLECULES
Volume 28, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/molecules28186517
Keywords
snake venom; secreted phospholipase A(2); stability; anti-tumor effect; cytotoxicity; human cancer cell lines
Ask authors/readers for more resources
This study identified and characterized a new type II secreted phospholipase A(2) with unique characteristics. The enzyme showed high activity and stability, and exhibited dose-dependent cytotoxic effects on various human cancer cell lines, while potentially having angiogenic activity as well.
Secreted phospholipases A(2) are snake-venom proteins with many biological activities, notably anti-tumor activity. Phospholipases from the same snake type but different geographical locations have shown similar biochemical and biological activities with minor differences in protein sequences. Thus, the discovery of a new phospholipase A(2) with unique characteristics identified in a previously studied venom could suggest the origins of these differences. Here, a new Group II secreted phospholipase A(2) (Cc-PLA(2)-II) from the snake venom of Saudi Cerastes cerastes gasperetti was isolated and characterized. The purified enzyme had a molecular weight of 13.945 kDa and showed high specific activity on emulsified phosphatidylcholine of 1560 U/mg at pH 9.5 and 50 degrees C with strict calcium dependence. Interestingly, stability in extreme pH and high temperatures was observed after enzyme incubation at several pH levels and temperatures. Moreover, a significant dose-dependent cytotoxic anti-tumor effect against six human cancer cell lines was observed with concentrations of Cc-PLA(2) ranging from 2.5 to 8 mu M. No cytotoxic effect on normal human umbilical-vein endothelial cells was noted. These results suggest that Cc-PLA(2)-II potentially has angiogenic activity of besides cytotoxicity as part of its anti-tumor mechanism. This study justifies the inclusion of this enzyme in many applications for anticancer drug development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available