Journal
MOLECULES
Volume 28, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/molecules28176219
Keywords
starch; starch-interacting proteins; glucan water dikinase; phosphoglucan water dikinase; plastidial phosphorylase; potato; Solanum tuberosum L.
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Proteomic approaches have revealed the interaction between potato starch granules and various proteins, most of which are involved in starch metabolism. The study also found that GWD and PWD, two dikinases associated with starch degradation, play an important role in storage starch synthesis. Additionally, there were differences in the starch-interacting protein profiles of transgenic and wild type tubers under different storage temperatures, indicating differential expression in response to environmental changes.
The complete mechanism behind starch regulation has not been fully characterized. However, significant progress can be achieved through proteomic approaches. In this work, we aimed to characterize the starch-interacting proteins in potato (Solanum tuberosum L. cv. Desiree) tubers under variable circumstances. Starch-interacting proteins were extracted from developing tubers of wild type and transgenic lines containing antisense inhibition of glucan phosphorylases. Further, proteins were separated by SDS-PAGE and characterized through mass spectrometry. Additionally, starch-interacting proteins were analyzed in potato tubers stored at different temperatures. Most of the proteins strongly interacting with the potato starch granules corresponded to proteins involved in starch metabolism. GWD and PWD, two dikinases associated with starch degradation, were consistently found bound to the starch granules. This indicates that their activity is not only restricted to degradation but is also essential during storage starch synthesis. We confirmed the presence of protease inhibitors interacting with the potato starch surface as previously revealed by other authors. Starch interacting protein profiles of transgenic tubers appeared differently from wild type when tubers were stored under different temperatures, indicating a differential expression in response to changing environmental conditions.
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