Analysis of Pork in Beef Sausages Using LC-Orbitrap HRMS Untargeted Metabolomics Combined with Chemometrics for Halal Authentication Study

Review Chemistry, Applied

Untargeted metabolomic approaches in food authenticity: A review that showcases biomarkers

Nicolas Mialon et al.

Summary: The assessment of food authenticity, especially geographic origin fraud, has gained international attention. Non-targeted analysis provides new solutions to detect unexpected situations and search for environmental terroir markers.

FOOD CHEMISTRY (2023)

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Article Food Science & Technology

Metabolomics and Proteomics Approach Using LC-Orbitrap HRMS for the Detection of Pork in Tuna Meat for Halal Authentication

Anjar Windarsih et al.

Summary: This study developed an untargeted proteomics and metabolomics approach using LC-HRMS to detect pork adulteration in tuna meat (TM). Metabolomics combined with PCA and PLS-DA could differentiate and detect pork in adulterated TM samples. Proteomics analysis successfully detected peptide markers of pork from myoglobin, carbonic anhydrase 3, and beta-haemoglobin. Both proteomics and metabolomics could detect up to 0.5% of pork concentration in TM.

FOOD ANALYTICAL METHODS (2023)

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Article Food Science & Technology

TaqMan multiplex qPCR for detecting animal species in meat and meat products: Development, recent advances and future prospects

M. A. Motalib Hossain et al.

Summary: Meat and meat products are highly sought after globally for their high-quality protein and nutrients, but the industry faces challenges such as food fraud, mislabeling, and zoonotic diseases. Quantitative detection of the species origin using methods like PCR is crucial for food control. This review focuses on the design, development, and applications of TaqMan probe-based mqPCR techniques for authenticating meat products, providing a reference for academia and industry to improve existing assays.

FOOD CONTROL (2023)

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Review Food Science & Technology

Authentication of Halal and Kosher meat and meat products: Analytical approaches, current progresses and future prospects

M. A. Motalib Hossain et al.

Summary: The authenticity of Halal and Kosher meat and meat products is a concern for consumers, and reliable and sensitive techniques are needed for authentication. This review provides a critical overview of the current progress, challenges, and methods used in analyzing animal species in meat products. It also discusses the slaughtering procedures and laws of Halal and Kosher, aiming to raise consumer awareness of fraudulent practices.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

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Article Chemistry, Medicinal

A Review on the Foodomics Based on Liquid Chromatography Mass Spectrometry

Yoshio Muguruma et al.

CHEMICAL & PHARMACEUTICAL BULLETIN (2022)

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Article Chemistry, Applied

UHPLC-Q-Orbitrap-based lipidomics reveals molecular mechanism of lipid changes during preservatives treatment of Hengshan goat meat sausages

Wei Jia et al.

Summary: The study revealed significant impact of preservatives on lipid composition of sausages, with natamycin showing better preservation and nutritional value compared to the other two preservatives. Major lipid variables were primarily associated with glycerophospholipid and sphingolipid metabolism.

FOOD CHEMISTRY (2022)

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Article Biochemical Research Methods

Current state-of-the-art of separation methods used in LC-MS based metabolomics and lipidomics

Eva-Maria Harrieder et al.

Summary: Metabolomics and lipidomics involve the large-scale analysis of metabolites, requiring advanced analytical methods. Liquid Chromatography-Mass Spectrometry (LC-MS) is a commonly used technique that provides different selectivities in separation and high sensitivity in detection. Due to the huge chemical diversity, there is no single analysis method that can cover the entire range of metabolites or lipids, leading to the use of different separation methods. This review explores the current use of LC-MS in metabolomics and lipidomics using data from public databases, and highlights potential future trends and improvements needed.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2022)

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Review Food Science & Technology

Recent advances in halal food authentication: Challenges and strategies

Pei Chi Ng et al.

Summary: The increasing public awareness of food quality and safety has led to a rapid growth in halal food authentication. Analytical methods are being researched extensively to ensure accurate and reliable results for monitoring and controlling the authenticity of halal food. The review provides an impartial overview of recent studies on analytical techniques for halal food authenticity, highlighting the need for advancements in technology and methodologies.

JOURNAL OF FOOD SCIENCE (2022)

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Article Food Science & Technology

Impact of irradiation on metabolomics profile of ground meat and its implications toward food safety

Sara Panseri et al.

Summary: This research used metabolomics approach to analyze the global metabolome profile of irradiated chicken, turkey, and mixed ground meat. The results showed that the metabolome profile of all three meat categories were not significantly affected by irradiation, and no relevant negative impact on meat safety was detected. The study highlights the usefulness of metabolomics in distinguishing irradiated meat from non-treated meat.

LWT-FOOD SCIENCE AND TECHNOLOGY (2022)

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Article Food Science & Technology

Non-volatile metabolite changes in low-temperature sausage stored at room temperature

Hongjiao Han et al.

Summary: This study aimed to evaluate changes in non-volatile metabolites in low-temperature sausage during storage using LC-MS/MS metabolomics. Significant differences were observed in 245 metabolites, indicating potential protein catabolism and incomplete beta-oxidation leading to quality deterioration. These findings enhance understanding of metabolic changes in low-temperature meat products and could aid in identifying chemical biomarkers for assessing meat quality.

FOOD PACKAGING AND SHELF LIFE (2022)

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Article Biochemistry & Molecular Biology

Detection of Pork in Beef Meatballs Using LC-HRMS Based Untargeted Metabolomics and Chemometrics for Halal Authentication

Anjar Windarsih et al.

Summary: This study aimed to develop a non-targeted metabolomics method using LC-HRMS and chemometrics to detect adulteration of pork in beef meatballs. The research showed that chemometrics techniques such as PCA, PLS-DA, and OPLS-DA could accurately differentiate between beef and pork meatballs and predict the presence of pork in beef meatballs with high accuracy.

MOLECULES (2022)

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Article Chemistry, Applied

Comprehensive identification of molecular profiles related to sensory and nutritional changes in Mongolian cheese during storage by untargeted metabolomics coupled with quantification of free amino acids

Xin Zhang et al.

Summary: Non-targeted metabolomics was used to study metabolites that may contribute to quality deterioration of Mongolian cheese during storage. The results showed that bitter amino acids, bitter peptide, and organic acids increased during storage, while overall acceptability decreased.

FOOD CHEMISTRY (2022)

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Review Food Science & Technology

Use of chromatographic-based techniques and chemometrics for halal authentication of food products: A review

Laela Hayu Nurani et al.

Summary: This review highlights the use of chromatographic methods in combination with chemometrics or multivariate data analysis for reliable separation capacity in halal authentication analysis, offering techniques to differentiate halal and non-halal samples, as well as for determination and quantification of halal and non-halal components in food products.

INTERNATIONAL JOURNAL OF FOOD PROPERTIES (2022)

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Article Food Science & Technology

Non-target metabolomics reveals the changes of small molecular substances in duck breast meat under different preservation time

Hehe Liu et al.

Summary: Poultry products are an essential source of protein for humans, but long-term frozen storage may affect the quality of meat products. This study used non-targeted metabolomics to reveal the changes in small molecular substances in duck meat during long-term storage. The results showed that as the storage time increased, the nitrogen-containing compounds, nucleotides, organic acids, and carbohydrates in duck meat increased. These small molecular substances can be used as biomarkers to detect deterioration in long-term stored duck meat. The main metabolic processes during the long-term storage of duck meat were protein catabolism, nucleotide catabolism, fat decomposition and oxidation, and carbohydrate decomposition.

FOOD RESEARCH INTERNATIONAL (2022)

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Article Food Science & Technology

Study of Meat Species Adulteration in Indonesian Commercial Beef Meatballs Related to Halal Law Implementation

Hamzah Nata Siswara et al.

Summary: This study aimed to detect the contamination of pork and chicken in commercial beef meatballs in the Indonesian market using the PCR method. The results showed that most samples were contaminated with chicken meat, and some commercial beef meatballs also contained pork. This indicates the presence of meat contamination that does not match the label in meatballs circulating in the Indonesian market.

FRONTIERS IN SUSTAINABLE FOOD SYSTEMS (2022)

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Article Biochemistry & Molecular Biology

Metabolite Fingerprinting Using 1H-NMR Spectroscopy and Chemometrics for Classification of Three Curcuma Species from Different Origins

Laela Hayu Nurani et al.

Summary: The study on the authentication of Curcuma species using H-1-NMR spectroscopy and chemometrics has demonstrated the importance of metabolite fingerprinting in the authentication of herbal and medicinal plants.

MOLECULES (2021)

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Article Multidisciplinary Sciences

Incidence of fraud and adulterations in ASEAN food/feed exports: A 20-year analysis of RASFF's notifications

Iyiola Oluwakemi Owolabi et al.

Summary: This study investigated the occurrence of food fraud and adulterations in exports from ASEAN countries, finding that seven out of the ten member countries had cases reported over a 20-year period. Thailand and the Philippines received the highest frequency of notifications, with the highest number of notifications coming from the United Kingdom. Border rejections and subsequent destruction of products were common, especially for exports from Indonesia.

PLOS ONE (2021)

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Review Chemistry, Analytical

Chemometric applications in metabolomic studies using chromatography-mass spectrometry

Alessandra Paul et al.

Summary: Metabolomic studies generate complex datasets, and chemometric methods are commonly used for analysis. Techniques can be categorized into statistical methods, machine learning models, and custom solutions, with researchers choosing the right approach based on experimental design and hypothesis. Multiple methods are often used in a pipeline for systematic data analysis.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2021)

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Review Chemistry, Analytical

Recent trends in application of chemometric methods for GC-MS and GCxGC-MS-based metabolomic studies

Neda Feizi et al.

Summary: Metabolomics is the study of metabolites in biological systems, utilizing chemometric methods to process data and extract valuable information from data generated by GC-MS and GC x GC-MS.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Volatilomics for halal and non-halal meatball authentication using solid-phase microextraction-gas chromatography-mass spectrometry

Agy Wirabudi Pranata et al.

Summary: This study utilized SPME/GC-MS and multivariate data analysis to differentiate volatile compounds in meatballs made from beef, chicken, and wild boar. The results demonstrated that this method was a fast and reliable way to distinguish between different types of meatballs based on their volatile compound contents.

ARABIAN JOURNAL OF CHEMISTRY (2021)

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Article Biochemistry & Molecular Biology

1H-NMR-Based Metabolomics: An Integrated Approach for the Detection of the Adulteration in Chicken, Chevon, Beef and Donkey Meat

Muhammad Tayyab Akhtar et al.

Summary: Meat is a rich source of nutrition and energy, with different types consumed globally to meet varying dietary requirements. This study successfully differentiated various meat samples based on their metabolite profiles using NMR spectroscopy and multivariate data analysis techniques. Key metabolites were identified that distinguish between different types of meat, showcasing the potential of NMR-based metabolomics as a tool for quality control and differentiation purposes in the meat industry.

MOLECULES (2021)

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Article Biochemical Research Methods