Journal
MOLECULES
Volume 28, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/molecules28186479
Keywords
MTT assay; cell toxicity; cytokine gene expression; nanoencapsulation; dynamic light scattering; topical formulation
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This study evaluated the toxicological effects of hemp essential oil (EO) and its nanoemulsion (NE) form on cell lines. The results showed that both EO and NE have comparable cytotoxicity and minimal impact on inflammation, supporting their safety for medical applications.
Industrial hemp (Cannabis sativa L.), due to its bioactive compounds (terpenes and cannabinoids), has gained increasing interest in different fields, including for medical purposes. The evaluation of the safety profile of hemp essential oil (EO) and its encapsulated form (nanoemulsion, NE) is a relevant aspect for potential therapeutic applications. This study aimed to evaluate the toxicological effect of hemp EOs and NEs from cultivars Carmagnola CS and Uso 31 on three cell lines selected as models for topical and inhalant administration, by evaluating the cytotoxicity and the cytokine expression profiles. Results show that EOs and their NEs have comparable cytotoxicity, if considering the quantity of EO present in the NE. Moreover, cells treated with EOs and NEs showed, in most of the cases, lower levels of proinflammatory cytokines compared to Etoposide used as a positive control, and the basal level of inflammatory cytokines was not altered, suggesting a safety profile of hemp EOs and their NEs to support their use for medical applications.
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