Journal
MOLECULAR IMMUNOLOGY
Volume 160, Issue -, Pages 112-120Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2023.06.009
Keywords
CD8; T cell; Repertoire; Vitiligo; High-throughput sequencing
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This study assessed the TCRβ repertoire diversity and composition of CD8+ T cells in blood of 9 nonsegmental vitiligo patients via high-throughput sequencing. Vitiligo patients showed low TCRβ diversity with highly expanded clones. Differential usage of TRBV, the TRBJ gene, and the TRBV/TRBJ combination were observed between vitiligo patients and healthy controls. A set of TRBV/TRBJ combinations could differentiate vitiligo patients from healthy controls. This study revealed distinct TCRβ repertoires of CD8+ T cells in vitiligo and may contribute to the exploration of immune biomarkers and therapeutic targets.
Vitiligo is an autoimmune depigmentation dermatosis induced by melanocyte destruction, and CD8+ T cells play a pivotal role in melanocyte destruction. However, an accurate profile of the CD8+ T cell receptor (TCR) repertoire in vitiligo patients has not been reported, and the clonotype features of the involved CD8+ T cells remain largely unknown. This study aimed to assess the TCRll chain repertoire diversity and composition of blood in nine nonsegmental vitiligo patients via high-throughput sequencing. Vitiligo patients manifested a low TCRll repertoire diversity with highly expanded clones. Differential usage of TRBV, the TRBJ gene, and the TRBV/TRBJ combination were compared between patients with vitiligo and healthy controls. A set of TRBV/ TRBJ combinations could differentiate patients with vitiligo from healthy controls (area under the curve = 0.9383, 95% CI: 0.8167-1.00). Our study revealed distinct TCRll repertoires of CD8+ T cells in patients with vitiligo and will help explore novel immune biomarkers and potential therapeutic targets for vitiligo.
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