4.5 Article

A novel method for age identification of mountain-cultivated ginseng

Journal

MICROSCOPY RESEARCH AND TECHNIQUE
Volume -, Issue -, Pages -

Publisher

WILEY
DOI: 10.1002/jemt.24393

Keywords

annual growth rings; cultivated ginseng (CG); mountain-cultivated ginseng (MCG); radius; ring density; secondary xylem; staining method

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A new method was established to determine the age of Panax ginseng, which is important for assessing its market value and medical effects.
Panax ginseng, a slow-growing perennial herb, is the most praised and popular traditional medicinal herb. Mountain-cultivated ginseng (MCG) and cultivated ginseng (CG) both belong to Panax ginseng C. A. Meyer. The market price and medical effects of this popular health product are closely related to its age. It is widely acknowledged that CG is typically harvested after 4-6 years of growth, but MCG is often collected after 10 years. Until now, the age identification of MCG or mountain wild ginseng (MWG) has remained a major challenge. In this study, we established a novel and rapid method for staining xylem vessels with phloroglucinol and identifying the annual growth rings of ginseng by utilizing a stereoscope, which serves as a reliable indicator of the age of MCG. Statistical analysis of the ring radius and the ring density of MCG aged from 1 to 20 years shows that the secondary xylem of MCG increases rapidly in the first 3 years but then gradually slows down from 4 to 10 years, and minor fluctuation is observed in the next 10 years. Meanwhile, the space between the growth rings (ring density) becomes increasingly small with age. This straightforward staining approach can reveal the age of MCG with remarkable clarity and can distinguish MCG from CG.

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