4.7 Article

Detection of receptor tyrosine kinase-orphan receptor-2 using an electrochemical immunosensor modified with electrospun nanofibers comprising polyvinylpyrrolidone, soy, and gold nanoparticles

Journal

MICROCHIMICA ACTA
Volume 190, Issue 10, Pages -

Publisher

SPRINGER WIEN
DOI: 10.1007/s00604-023-06002-8

Keywords

Receptor tyrosine kinase-orphan receptor-2; Immunosensor; Monoclonal antibody; Electrospun nanofibers; Modified glassy carbon electrode; Differential pulse voltammetry; PVP/soy/AuNPs

Ask authors/readers for more resources

An electrochemical immunosensing platform was developed using a modified glassy carbon electrode and nanomaterials for the detection of receptor tyrosine kinase-orphan receptor-2 (ROR2). The modified nanomaterials exhibited good electrochemical behavior, mechanical strength, and sensitivity. Through various characterization methods, the designed immunosensor performance was validated and showed accurate detection of ROR2 with good selectivity, reproducibility, and stability in human plasma samples.
An electrochemical immunosensing platform was developed for the detection of receptor tyrosine kinase-orphan receptor-2 (ROR2) at a glassy carbon electrode (GCE) modified with the electrospun nanofiber containing polyvinylpyrrolidone (PVP), soy, and Au nanoparticles (AuNPs). The PVP/soy/AuNP nanofiber exhibited good electrochemical behavior due to synergistic effects between PVP, soy, and AuNPs. The PVP/soy in the modified film provided good mechanical strength, high porosity, flexible structures, and high specific surface area. On the other hand, the presence of AuNPs effectively improved conductivity, as well as the immobilization of anti-ROR2 on the modified GCE, leading to enhanced sensitivity. Various characterization approaches such as FE-SEM, FTIR, and EDS were used for investigating the morphological and structural features, and the elemental composition. The designed immunosensor performance was investigated using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV). Under optimum conditions with a working potential range from -0.2 to 0.6 V (vs. SCE), sensitivity, linear range (LR), limit of detection (LOD), and correlation coefficient (R-2) were acquired at 122.26 mu A/cm(2) dec, 0.01-1000 pg/mL, 3.39 fg/mL, and 0.9974, respectively. Furthermore, the determination of ROR2 in human plasma samples using the designed immunosensing platform was examined and exhibited satisfactory results including good selectivity against other proteins, reproducibility, and cyclic stability.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available