One-pot wet-chemical fabrication of 3D urchin-like core-shell Au@PdCu nanocrystals for electrochemical breast cancer immunoassay

Carbohydrate antigen 15-3 (CA15-3) is an important biomarker for early diagnosis of breast cancer. Herein, a label-free electrochemical immunosensor was built based on three-dimensional (3D) urchin-like core-shell Au@PdCu nanocrystals (labeled Au@PdCu NCs) for highly sensitive detection of CA15-3, where K-3[Fe(CN)(6)] behaved as an electroactive probe. The Au@PdCu NCs were synthesized by a simple one-pot wet-chemical approach and the morphology, structures, and electrocatalytic property were investigated by several techniques. The Au@PdCu NCs prepared worked as electrode material to anchor more antibodies and as signal magnification material by virtue of its exceptional catalytic property. The developed biosensor exhibited a wide linear detection range from 0.1 to 300 U mL(-1) and a low limit of detection (0.011 U mL(-1), S/N = 3) for determination of CA15-3 under the optimal conditions. The established biosensing platform exhibits some insights for detecting other tumor biomarkers in clinical assays and early diagnosis.

Automated summary

Carbohydrate antigen 15-3 (CA15-3) is an important biomarker for early diagnosis of breast cancer. In this study, a label-free electrochemical immunosensor was built using urchin-like core-shell Au@PdCu nanocrystals (labeled Au@PdCu NCs) for highly sensitive detection of CA15-3. The Au@PdCu NCs synthesized by a simple one-pot wet-chemical approach demonstrated exceptional catalytic properties for anchoring more antibodies and amplifying the signal. The developed biosensor exhibited a wide linear detection range and a low limit of detection for determination of CA15-3 under the optimal conditions.