A terpyridyl functionalized rhodamine dye for viscosity sensing with fluorescence enhancement and mitochondria-targeting imaging

Exploring new fluorescent organic dyes with mitochondria-targeted and viscosity-sensitive functions attracts extensive attention because they may provide access to a better understanding of the microenvironments of the subcellular level via biological imaging. Herein a terpyridyl functionalized rhodamine dye (TRMe) has been facilely designed and prepared, which shows a highly selective fluorescence enhancement response to viscosity (1.0-1238.0 cp) in water-glycerol mixtures with the increased glycerol content. In addition, TRMe can respond to viscosity enhancement over a wide pH range (1.0-10.0) and presents low cell toxicity. Besides, a good linearity between the viscosity and fluorescence lifetime of TRMe can be observed. Significantly, TRMe shows excellent mitochondria-targeted performance with a Pearson's colocalization coefficient of 0.958 and can be employed as a tracer for viscosity imaging in Nystatin-stimulated living HeLa cells.

Automated summary

In this study, a terpyridyl functionalized rhodamine dye (TRMe) was designed and prepared, which selectively enhances fluorescence response to viscosity in water-glycerol mixtures and can respond to viscosity enhancement over a wide pH range. Furthermore, TRMe demonstrates excellent mitochondria-targeted performance and can be used as a tracer for viscosity imaging in living cells.