Journal
MOLECULAR MEDICINE REPORTS
Volume 13, Issue 3, Pages 1967-1974Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2016.4769
Keywords
nonsyndromic hearing impairment; gap junction protein 2; mitochondrial DNA; solute carrier family 26; member 4; gene diagnosis; genetic counseling
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Funding
- Program for Leading Talents and Medical Innovative Team of Jiangsu Province [LJ201109]
- Special Funds of Clinical Medicine Science and Technology of Jiangsu Province [BL2012039]
- Science and Technology Research and Development Program of Health Bureau of Nanjing City [YKK11059]
- Training Program for Young Talents of Nanjing City
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The aim of the present study was to investigate the genetic etiology of patients with nonsyndromic hearing impairment through gene analysis, and provide accurate genetic counseling and prenatal diagnosis for deaf patients and families with deaf children. Previous molecular etiological studies have demonstrated that the most common molecular changes in Chinese patients with nonsyndromic hearing loss (NSHL) involved gap junction protein 2, solute carrier family 26, member 4 (SLC26A4), and mitochondrial DNA 12S rRNA. A total of 117 unrelated NSHL patients were included. Mutation screening was performed by Sanger sequencing in GJB2, 12S rRNA, and the hot-spot regions of SLC26A4. In addition, patients with a single mutation of SLC26A4 in the hot-spot regions underwent complete exon sequencing to identify a mutation in the other allele. A total of 36 of the 117 deaf patients were confirmed to have two pathogenic mutations, which included 4 deaf couples, husband or wife in 11 deaf couples and 17 deaf individuals. In addition, prenatal diagnoses was performed in 7 pregnant women at 18-21 weeks gestation who had previously given birth to a deaf child, and the results showed that two fetal genotypes were the same as the proband's genotypes, four fetuses carried one pathogenic gene from their parents, and one fetus was identified to have no mutations. Taken together, the genetic testing of deaf patients can provide reasonable guidance to deaf patients and families with deaf children.
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