Journal
MOLECULAR MEDICINE REPORTS
Volume 13, Issue 5, Pages 3993-4000Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2016.5030
Keywords
U83836E; ischemia/reperfusion; oxidative stress; protein kinase C; mitochondria
Categories
Funding
- Natural Science Foundation of Shanxi Province [2009011055-3]
- Key Displine Construction Special Fund Projects grant from the Shanxi Province Colleges and Universities [20131007]
- Science and Technology Innovation Team Project from Changzhi Medical College [CX201409]
Ask authors/readers for more resources
Oxidative stress has been demonstrated to be important during myocardial ischemia/reperfusion injury (MIRI). The lazaroid U83836E, which combines the amino functionalities of the 21-aminosteroids with the antioxidant ring portion of vitamin E, is a reactive oxygen species scavenger. The aim of the current study was to investigate the effect of U83836E on MIRI and its mechanisms of action. Rat hearts were subjected to 30 min ligation of the left anterior descending coronary artery, followed by 2 h reperfusion. The results demonstrated that at 5 mg/kg, U83836E markedly protected cardiac function in ischemia/reperfusion rat models, decreased the malondialdehyde content and creatinine kinase activity, while increasing superoxide dismutase and glutathione peroxidase activity. Additionally, U83836E significantly decreased the histological damage to the myocardium, reduced the area of myocardial infarction in the left ventricle and modified the mitochondrial dysfunction. Furthermore, U83836E enhanced the translocation of protein kinase C epsilon (PKC epsilon) from the cytoplasm to the membrane. However, the cardioprotective effects of U83836E were reduced in the presence of the PKC inhibitor, chelerythrine (1 mg/kg). Therefore, the results of the present study suggest that U83836E has a potent protective effect against MIRI in rat models through the direct anti-oxidative stress mechanisms and the activation of PKC signaling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available