Journal
MOLECULAR IMAGING AND BIOLOGY
Volume 18, Issue 4, Pages 473-478Publisher
SPRINGER
DOI: 10.1007/s11307-016-0934-0
Keywords
Superoxide anion; Reactive oxygen species; Inflammatory bowel disease; Coelenterazine; Imaging
Funding
- Chamber's Family Foundation for Excellence in Pediatric Research
- Children's Health Research Institute at Stanford University
Ask authors/readers for more resources
Reactive oxygen species (ROS) are important contributors to inflammatory bowel disease (IBD); however, there are insufficient tools for their in vivo evaluation. To determine if a chemiluminescent ROS reporter, coelenterazine, would be a useful tool for the detection of immune cell activation, the macrophage cell line (RAW 264.7) was treated with phorbol myristate acetate (PMA). Additionally, coelenterazine was used to monitor the changes in ROS production over time in a mouse model of IBD. In vitro, coelenterazine enabled the dynamic monitoring of the RAW 264.7 cell oxidative burst. In vivo, there were early, preclinical, changes in the localization and magnitude of coelenterazine chemiluminescent foci. Coelenterazine offers a high-throughput method for assessing immune cell activation in culture and provides a means for the in vivo detection and localization of ROS during IBD disease progression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available