Molecular precursors to produce para-hydrogen enhanced metabolites at any field

Enhancing magnetic resonance signal via hyperpolarization techniques enables the real-time detection of metabolic transformations even in vivo. The use of para-hydrogen to enhance 13C-enriched metabolites has opened a rapid pathway for the production of hyperpolarized metabolites, which usually requires specialized equipment. Metabolite precursors that can be hyperpolarized and converted into metabolites at any given field would open up opportunities for many labs to make use of this technology because already existing hardware could be used. We report here on the complete synthesis and hyperpolarization of suitable precursor molecules of the side-arm hydrogenation approach. The better accessibility to such side-arms promises that the para-hydrogen approach can be implemented in every lab with existing two channel NMR spectrometers for 1H and 13C independent of the magnetic field. The use of para-hydrogen to enhance 13C-enriched metabolites has opened a rapid pathway for the production of hyperpolarized metabolites. Precursors that can be hyperpolarized and converted into metabolites at any given field would open up opportunities for many labs to make use of this technology. We report on the synthesis and hyperpolarization of suitable precursor molecules of the sidearm hydrogenation approach for implementation in any existing two channel NMR spectrometers for 1H and 13C independent of the magnetic field.image

Automated summary

The use of para-hydrogen to enhance 13C-enriched metabolites has provided a rapid pathway for producing hyperpolarized metabolites, which can be achieved at any given field. This opens up opportunities for many laboratories to utilize this technology.