Multiplex TaqMan real-time PCR assay for high-throughput identification of highly toxic mushroom species-induced foodborne poisoning

Article Toxicology

Rapid detection of α-amanitin and β-amanitin in rat plasma by ultra-performance liquid chromatography-tandem mass spectrometry and its application to the toxicokinetic study of Lepiota brunneoincarnata

Zhengsheng Mao et al.

Summary: In this study, an UPLC-MS/MS method was developed for quantifying amanitines in rat plasma, enabling the toxicokinetic study of Lepiota brunneoincarnata extracts.

FORENSIC TOXICOLOGY (2022)

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Article Fisheries

Development and application of TaqMan probe-based quantitative PCR assays for the detection of tilapia parvovirus

Jidapa Yamkasem et al.

Summary: A new TaqMan probe-based quantitative PCR assay was developed for detecting Tilapia parvovirus, showing good repeatability and reproducibility with a detection limit of 10 copies/mu l. The assays had comparable sensitivity to a previously developed SYBR Green qPCR and were more sensitive than conventional PCR, without cross-reactivity with other viruses and bacteria. These assays offer high sensitivity and specificity in detecting low concentrations of TiPV DNA in infected tilapia samples, providing a valuable diagnostic tool for experimental and field samples.

JOURNAL OF FISH DISEASES (2022)

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Article Toxicology

An outbreak of mushroom poisoning in Israel during the 2020 fall and winter season: an unexpected outcome of COVID-19 restrictions?

Yael Lurie et al.

Summary: In the fall/winter of 2020, Israel experienced a significant increase in mushroom poisonings, with 105 calls to the Israel Poison Information Center (IPIC) compared to previous years. Most cases were mild, but 6% resulted in moderate to severe poisoning, including one life-threatening case caused by ingestion of Lepiota brunneoincarnata and Amanita proxima. Possible reasons for this outbreak include favorable climate conditions and increased outdoor activities during the COVID-19 pandemic.

CLINICAL TOXICOLOGY (2022)

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Article Chemistry, Applied

A Rapid colorimetric assay for field-portable authentication of Amanita sepiacea using the beta-tubulin gene sequence

Jie Gao et al.

Summary: A colorimetric method based on LAMP technology was established in this study for rapid detection of toxic mushroom Amanita sepiacea. The method showed strong specificity, simple operation, and can detect A. sepiacea at the lowest concentration in mixed mushroom samples.

JOURNAL OF FOOD COMPOSITION AND ANALYSIS (2022)

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Article Parasitology

Establishment and application of a TaqMan probe-based qPCR for the detection of Enterocytozoon hepatopenaei in shrimp Litopenaeus vannamei

Wei-Feng Chen et al.

Summary: This study established a TaqMan probe-based qPCR method for quantitative detection of Enterocytozoon hepatopenaei (EHP) in both shrimp and aquatic environment samples, which showed higher sensitivity and efficiency in clinical sample detection.

PARASITOLOGY RESEARCH (2022)

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Article Public, Environmental & Occupational Health

Russula subnigricans Poisoning Causes Severe Rhabdomyolysis That Could be Misdiagnosed as Non-ST Segment Elevation Myocardial Infarction

Mun Ki Min et al.

Summary: The number of patients visiting emergency rooms due to mushroom poisoning and the proportion of fatal poisonings are increasing. This report presents a case of a family with Russula subnigricans poisoning complicated by severe rhabdomyolysis, including a misdiagnosed case of myocardial infarction. Early recognition and intensive supportive care are important for mushroom poisoning patients.

WILDERNESS & ENVIRONMENTAL MEDICINE (2022)

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Article Chemistry, Applied

Ultrasensitive paper sensor for simultaneous detection of alpha-amanitin and beta-amanitin by the production of monoclonal antibodies

Shengyang Zhou et al.

Summary: This study constructed a gold nanoparticle-based immunosensor for rapid detection of amanitin in mushroom samples. The results showed that this method had high sensitivity and accuracy, allowing for detection within 10 minutes. It can be widely used in food safety monitoring and clinical diagnosis.

FOOD CHEMISTRY (2022)

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Article Microbiology

Development of a loop-mediated isothermal amplification assay for the rapid detection of Russula subnigricans and Russula japonica

Pan Long et al.

Summary: The study aimed to develop a rapid, specific, sensitive, and simple LAMP assay for the detection of R. subnigricans and R. japonica. The results showed that LAMP had high specificity and sensitivity, and was faster than PCR.

FRONTIERS IN MICROBIOLOGY (2022)

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Article Green & Sustainable Science & Technology

Molecular Identification of Mushroom Species in Italy: An Ongoing Project Aimed at Reinforcing the Control Measures of an Increasingly Appreciated Sustainable Food

Alice Giusti et al.

Summary: Proper investment in mushroom production can help address food security and environmental impact issues, while enhancing the quality of local products. However, consumption of poisonous species poses risks that require effective government control measures. Genotypic analysis is a viable alternative for mushroom identification in cases where phenotype observation is not feasible.

SUSTAINABILITY (2021)

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Article Microbiology

Rapid and Visual Identification of Chlorophyllum molybdites With Loop-Mediated Isothermal Amplification Method

Nan Wang et al.

Summary: A loop-mediated isothermal amplification (LAMP) assay for visual detection of Chlorophyllum molybdites was established, offering an accurate, sensitive, rapid, and cost-effective method. The method can detect as low as 1% of C. molybdites samples, and results can be observed within 45 minutes based on a color change visible to the naked eye under daylight.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Microbiology

Exploring the Species Diversity of Edible Mushrooms in Yunnan, Southwestern China, by DNA Barcoding

Ying Zhang et al.

Summary: The study analyzed 3585 mushroom samples collected from wild mushroom markets in 35 counties across Yunnan Province, discovering 159 known species and 20 new species. The data indicates a rich diversity of mushroom species in Yunnan's wild mushroom markets, with many potential new taxa present.

JOURNAL OF FUNGI (2021)

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Article Public, Environmental & Occupational Health

Mushroom Poisoning Outbreaks - China, 2020

Haijiao Li et al.

Summary: This report provides an overview of mushroom poisonings in China, highlighting newly discovered toxic mushroom species and the different types of poisoning they can cause. It emphasizes the importance of promoting knowledge about safe consumption of mushrooms and the risks associated with consuming wild mushrooms, especially in southwestern provinces like Yunnan.

CHINA CDC WEEKLY (2021)

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Article Chemistry, Applied