Journal
MOLECULAR CELL
Volume 63, Issue 3, Pages 371-384Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2016.05.036
Keywords
-
Categories
Funding
- European Research Council
- Francis Crick Institute
- Cancer Research UK [19343, 10748, 15656] Funding Source: researchfish
- Cancer Research UK
- The Francis Crick Institute [10065] Funding Source: researchfish
- The Francis Crick Institute [10198, 10403, 10155] Funding Source: researchfish
Ask authors/readers for more resources
DNA replication during S phase is accompanied by establishment of sister chromatid cohesion to ensure faithful chromosome segregation. The Eco1 acetyltransferase, helped by factors including Ctf4 and Chl1, concomitantly acetylates the chromosomal cohesin complex to stabilize its cohesive links. Here we show that Ctf4 recruits the Chl1 helicase to the replisome via a conserved interaction motif that Chl1 shares with GINS and polymerase a. We visualize recruitment by EM analysis of a reconstituted Chl1-Ctf4-GINS assembly. The Chl1 helicase facilitates replication fork progression under conditions of nucleotide depletion, partly independently of Ctf4 interaction. Conversely, Ctf4 interaction, but not helicase activity, is required for Chl1's role in sister chromatid cohesion. A physical interaction between Chl1 and the cohesin complex during S phase suggests that Chl1 contacts cohesin to facilitate its acetylation. Our results reveal how Ctf4 forms a replisomal interaction hub that coordinates replication fork progression and sister chromatid cohesion establishment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available