Journal
MOLECULAR CELL
Volume 61, Issue 5, Pages 774-787Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2016.02.014
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Funding
- American Cancer Society Research Scholar Award
- NIH [R01GM111907, T32CA00910937]
- University of Virginia
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Centromeres are specialized chromatin domains specified by the centromere-specific CENP-A nucleosome. The stable inheritance of vertebrate centromeres is an epigenetic process requiring deposition of new CENP-A nucleosomes by HJURP. We show HJURP is recruited to centromeres through a direct interaction between the HJURP centromere targeting domain and the Mis18 alpha-beta C-terminal coiled-coil domains. We demonstrate Mis18 alpha and Mis18 beta form a heterotetramer through their C-terminal coiled-coil domains. Mis18 alpha-beta heterotetramer formation is required for Mis18BP1 binding and centromere recognition. S. pombe contains a single Mis18 isoform that forms a homotetramer, showing tetrameric Mis18 is conserved from fission yeast to humans. HJURP binding disrupts the Mis18 alpha-beta heterotetramer and removes Mis18a from centromeres. We propose stable binding of Mis18 to centromeres in telophase licenses them for CENP-A deposition. Binding of HJURP deposits CENP-A at centromeres and facilitates the removal of Mis18, restricting CENP-A deposition to a single event per cell cycle.
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