Journal
MOLECULAR CANCER THERAPEUTICS
Volume 15, Issue 5, Pages 1082-1094Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1535-7163.MCT-15-0707
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Funding
- National Natural Science Foundation of China [81272601, 81472198]
- Key Clinical Medicine Technology Foundation of Jiangsu Province [BL2014096]
- Medical Key Talented Person Foundation of the Jiangsu Provincial Developing Health Project [RC2011080]
- Innovation Team Project of the Second Affiliated Hospital of Nanjing Medical University [CX201202]
- 333 High Class Talented Man Project [2011-III-2630]
- School Key Fund of Nanjing Medical University [2013NJMU054]
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Long noncoding RNAs (lncRNA) are a novel class of transcripts with no protein coding capacity, but with diverse functions in cancer cell proliferation, apoptosis, and metastasis. The lncRNA PVT1 is 1,716 nt in length and located in the chr8q24.21 region, which also contains the myelocytomatosis (MYC) oncogene. Previous studies demonstrated that MYC promotes PVT1 expression in primary human cancers. However, the expression pattern and potential biologic function of PVT1 in non-small cell lung cancer (NSCLC) is still unclear. Here, we found that PVT1 was upregulated in 105 human NSCLC tissues compared with normal samples. High expression of PVT1 was associated with a higher tumor-node-metastasis stage and tumor size, as well as poorer overall survival. Functional analysis revealed that knockdown of PVT1 inhibited NSCLC cell proliferation and induced apoptosis both in vitro and in vivo. RNA immunoprecipitation and chromatin immunoprecipitation assays demonstrated that PVT1 recruits EZH2 to the large tumor suppressor kinase 2 (LATS2) promoter and represses LATS2 transcription. Furthermore, ectopic expression of LATS2 increased apoptosis and repressed lung adenocarcinoma cell proliferation by regulating the Mdm2-p53 pathway. Taken together, our findings indicated that PVT1/EZH2/LATS2 interactions might serve as new target for lung adenocarcinoma diagnosis and therapy. (C) 2016 AACR.
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