Optimizing the degradation of aflatoxin B1 in corn by Trametes versicolor and improving the nutritional composition of corn

BACKGROUND: Corn, being an important grain, is prone to contamination by aflatoxin B1 (AFB1), and AFB1-contaminated corn severely endangers the health of humans and livestock. Trametes versicolor, a fungus that can grow in corn, possesses the ability to directly degrade AFB1 through its laccase. This study aimed to optimize the fermentation conditions for T. versicolor to degrade AFB1 in corn and investigate the effect of T. versicolor fermentation on the nutritional composition of corn. AFB1contaminated corn was used as the culture substrate for T. versicolor. A combination of single-factor experiments and response surface methodology was employed to identify the optimal conditions of AFB1 degradation. RESULTS: The optimal conditions of AFB1 degradation were as follows: 9 days of fermentation, a fermentation temperature of 26.7 degrees C, a moisture content of 70.5% and an inoculation amount of 4.9 mL (containing 51.99 mg of T. versicolor mycelia). With the optimal conditions, the degradation rate of AFB1 in corn could reach 93.01%, and the dry basis content of protein and dietary fiber in the fermented corn was significantly increased. More importantly, the lysine content in the fermented corn was also significantly increased. CONCLUSION: This is the first report that direct fermentation of AFB1-contaminated corn by T. versicolor not only efficiently degrades AFB1 but also improves the nutritional composition of corn. These findings suggest that the fermentation of corn by T. versicolor is a promising, environmentally friendly and efficient approach to degrade AFB1 and improve the nutritional value of corn. (c) 2023 Society of Chemical Industry.

Automated summary

This study investigated the fermentation conditions for Trametes versicolor to degrade aflatoxin B1 (AFB1) in corn and its effect on the nutritional composition of corn. The optimal conditions for AFB1 degradation were determined, and the fermentation of AFB1-contaminated corn by T. versicolor was found to not only efficiently degrade AFB1 but also improve the nutritional value of corn. These findings suggest that the fermentation of corn by T. versicolor is a promising, environmentally friendly, and efficient approach to degrade AFB1 and improve the nutritional value of corn.