Noncovalent Peptide Assembly Enables Crystalline, Permutable, and Reactive Thiol Frameworks

Though thiols are exceptionally versatile, their high reactivity has also hindered the synthesis and characterization of well-defined thiol-containing porous materials. Leveraging the mild conditions of the noncovalent peptide assembly, we readily synthesized and characterized a number of frameworks with thiols displayed at many unique positions and in several permutations. Importantly, nearly all assemblies were structurally determined using single-crystal X-ray diffraction to reveal their rich sequence-structure landscape and the cooperative noncovalent interactions underlying their assembly. These observations and supporting molecular dynamics calculations enabled rational engineering by the positive and negative design of noncovalent interactions. Furthermore, the thiol-containing frameworks undergo diverse single-crystal-to-single-crystal reactions, including toxic metal ion coordination (e.g., Cd2+, Pb2+, and Hg2+), selective uptake of Hg2+ ions, and redox transformations. Notably, we find a framework that supports thiol-nitrosothiol interconversion, which is applicable for biocompatible nitric oxide delivery. The modularity, ease of synthesis, functionality, and well-defined nature of these peptide-based thiol frameworks are expected to accelerate the design of complex materials with reactive active sites.

Automated summary

By utilizing noncovalent peptide assembly, we have successfully synthesized and characterized various thiols-containing frameworks with different positions and permutations. The structures of these frameworks were determined using single-crystal X-ray diffraction, revealing their sequence-structure landscape and the cooperative noncovalent interactions involved in their assembly. These peptide-based thiol frameworks have diverse functionalities and undergo single-crystal-to-single-crystal reactions, making them suitable for various applications including metal ion coordination and redox transformations.