4.4 Article

The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane

Journal

MOLECULAR BIOLOGY OF THE CELL
Volume 27, Issue 24, Pages 3883-3893

Publisher

AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E16-08-0586

Keywords

-

Categories

Funding

  1. MEXT KAKENHI grant [JP23113714]
  2. JSPS KAKENHI Grant [JP26460065, JP15H01320, JP16H00764]
  3. Takeda Science Foundation
  4. Research Foundation for Pharmaceutical Sciences
  5. Grants-in-Aid for Scientific Research [16H00764, 15K07929, 26460065, 15H04370, 15K14456, 15H01320, 15H01211] Funding Source: KAKEN

Ask authors/readers for more resources

Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane trafficking; however, roles of mammalian P4-ATPases in membrane trafficking are poorly understood. We previously reported that ATP9A, one of 14 human P4-ATPases, is localized to endosomal compartments and the Golgi complex. In this study, we found that ATP9A is localized to phosphatidylserine (PS)-positive early and recycling endosomes, but not late endosomes, in HeLa cells. Depletion of ATP9A delayed the recycling of transferrin from endosomes to the plasma membrane, although it did not affect the morphology of endosomal structures. Moreover, depletion of ATP9A caused accumulation of glucose transporter 1 in endosomes, probably by inhibiting their recycling. By contrast, depletion of ATP9A affected neither the early/late endosomal transport and degradation of epidermal growth factor (EGF) nor the transport of Shiga toxin B fragment from early/recycling endosomes to the Golgi complex. Therefore ATP9A plays a crucial role in recycling from endosomes to the plasma membrane.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available