4.7 Article

Preparation of leaf protoplasts from Populus (Populus×xiaohei T. S. Hwang et Liang)and establishment of transient expression system

Related references

Note: Only part of the references are listed.
Article Plant Sciences

Protoplast-Based Transient Expression and Gene Editing in Shrub Willow (Salix purpurea L.)

Brennan Hyden et al.

Summary: This study presents an efficient method for isolating protoplasts from S. purpurea leaf tissue and demonstrates successful transient overexpression and CRISPR-Cas9 mediated mutations. This work holds significance for functional genomics studies in shrub willows.

PLANTS-BASEL (2022)

Article Biochemistry & Molecular Biology

Carrot DcALFIN4 and DcALFIN7 Transcription Factors Boost Carotenoid Levels and Participate Differentially in Salt Stress Tolerance When Expressed in Arabidopsis thaliana and Actinidia deliciosa

Luis Felipe Quiroz-Iturra et al.

Summary: ALFIN-like transcription factors (ALs) play important roles in physiological processes in plants, and the functional expression of DcAL4 and DcAL7 in carrot contributes to salt tolerance in Arabidopsis and kiwi plants.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Biology

Selection and functional identification of Dof genes expressed in response to nitrogen in Populus simonii x Populus nigra

Shenmeng Wang et al.

Summary: The study investigated the expression and function of Dof genes in poplar in response to nitrogen form and concentration. It identified 44 Dof genes, with some expressed under ammonia- and nitrate-N treatments. Overexpression of the PnDof30 gene in Arabidopsis thaliana resulted in improved growth and increased levels of carbon and nitrogen metabolism under low-N conditions. The findings suggest the biological significance of poplar Dof transcription factors in nitrogen response and regulation of downstream gene expression, providing insights into efficient nitrogen uptake and utilization mechanisms in trees.

OPEN LIFE SCIENCES (2022)

Article Cardiac & Cardiovascular Systems

Case Report: BMPR2-Targeted MinION Sequencing as a Tool for Genetic Analysis in Patients With Pulmonary Arterial Hypertension

Tomoya Takashima et al.

Summary: The study demonstrated the feasibility of using MinION nanopore sequencer for genetic analysis of PAH patients, particularly in detecting BMPR2 mutations. The portable nanopore sequencer showed promise as a screening tool for BMPR2 mutations, especially in small laboratories and research groups.

FRONTIERS IN CARDIOVASCULAR MEDICINE (2021)

Article Plant Sciences

Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops

Rui Ren et al.

Summary: The study established an efficient orchid protoplast isolation protocol by selecting suitable source materials and optimizing enzymatic conditions. Viable protoplasts were successfully isolated from different tissues and organs of orchids, showing marked advantages over previous protocols. The protoplast system was applied for transient expression and demonstrated high efficiency for further molecular studies in orchids and other economically important monocot crops.

FRONTIERS IN PLANT SCIENCE (2021)

Article Biochemical Research Methods

Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.)

S. V. G. N. Priyadarshani et al.

PLANT METHODS (2018)

Article Biochemical Research Methods

A simple improved-throughput xylem protoplast system for studying wood formation

Ying-Chung Lin et al.

NATURE PROTOCOLS (2014)

Article Biochemical Research Methods

Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis

Sang-Dong Yoo et al.

NATURE PROTOCOLS (2007)